摘要
采用N_6培养基为基本培养基,附加不同水平的生长素,培养普通小麦、小麦雄性不育系及其保持系共10个材料的未授粉子房,所用的材料都能诱导出愈伤组织,出愈率在4.2—38.1％,经分化培养分别从T型不育系和太谷核不育材料获得了单倍体植株,供试材料的基因型、培养基中的生长素的种类及用量,对诱导愈伤组织及分化绿苗有重要的作用。
The unpollinated ovaries of male sterile lines and its manitainers of wheat,as well as common wheat,were cultured on N6 medium in vitro,when the pollen of the same panicle were at uninucleate stage.Callus were obtained from all of the materials,haploid plantlets were regenerated from the unpollinated ovaries of timopheevi cytoplasm male sterile line(T75-3369A) and Taigu nucleus male sterile line(Tal×C1684).
出处
《遗传》
CAS
CSCD
北大核心
1993年第1期20-23,共4页
Hereditas（Beijing)
关键词
末授粉子房
小麦
三系配套
单倍体
Male sterile lines of wheat
Unpollinated ovaries
Haploid plants
