摘要
目的 探讨持续吸入高浓度氧对新生大鼠发育中肺的影响。方法 新生鼠生后分别于 85 %以上氧和空气中持续暴露 ,于 1、3、7、14、2 1d ,动态观察肺组织病理学改变和胶原染色面积的变化 ;肺组织SOD活性和MDA含量。结果 与空气对照组比较 ,高氧组肺泡发育过程受阻 ,肺损伤逐渐加重 ,最终纤维化 ;胶原染色阳性面积 7d时高于对照组 ,随时间延长而逐渐增加 ;MDA含量在吸氧 3d时升高 ,7d时最高 ,以后下降 ;SOD活性的改变较迟 ,7d时增高 ,持续增加 ,2 1d仍高于对照。结论 高氧可引起肺的氧化损伤 ,使新生鼠肺发育受阻 ,最终出现胶原增加的纤维化改变。
Objective: To determine the effect of prolonged hyperoxia on neonatal rat lung. Methods: Full-term newborn rats were continuously exposed to oxygen (>85% O 2) or room air (21% O 2) after birth. Lung histological change, collagen area; superoxide dismutase (SOD) activity and concentration of malondialdehyde (MDA) in the lung were monitered on days 1, 3, 7, 14 and 21 in hyperoxia groups and controls. Results: Compared with air group, the arrest of lung development was accompanied by interstitial fibrosis and increased collagen deposition, which was evident after 7d of oxygen exposure, increasing through 21d; Level of MDA in the lung was increased at 3d and reached a peak at 7d, then decreased. But SOD activities changed a little later with an increase at 7d, continuously to 21d. Conclusion: With prolonged hyperoxia exposure, the oxidant injury appears to lead to an arrest of lung development in neonatal rats and lung fibrosis with the increase of collagen.
出处
《中国优生与遗传杂志》
2004年第6期78-79,84,共3页
Chinese Journal of Birth Health & Heredity
基金
辽宁省教育厅高等学校科学研究项目
