摘要
豆类的贮存蛋白是人类的植物蛋白主要来源之一,稻米平均蛋白质含量只有8％,而且人类必需的赖氨酸含量较少。如果将较富赖氨酸的豆类贮存蛋白基因转移到水稻中,提高稻米的蛋白质和赖氨酸等氨基酸的含量,是改善米质的重要途径之一。 Sun首先从法国菜豆中分离出菜豆贮存蛋白基因(Gl),随后。
PCR amplification of legA gene form total DNA extract of pea seedlingis reported in this paper. The total pea DNA extract was obtained from frozen pea seedling, and was taken as the template for PCR amplification of legA gene, A pair of start and halt primers were synthesized. After 30 cycles of PCR amplification, a DNA fragment of 2. 0 kb was obtaind. Two DNA fragments of 0. 75 kb and J. 25 kb were obtained after the DNA segment was digested with BglI. The result demonstrates that product of the PCR amplification is pea legA Gene.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1993年第4期512-516,共5页
Journal of Xiamen University：Natural Science
