摘要
针对目前国内甜瓜的主栽品种K96 8× 6 1、T 5×red1 2 8和 94× 8 1的自交系及杂交种种子醇溶贮藏蛋白进行反相HPLC法分离。结果表明 ,其色谱图明显不同 ,可以用于该品种 (系 )的鉴定 ,认为RP HPLC技术是一种快速、准确、可信的甜瓜品种鉴定手段。
Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) of cereal grain proteins has been receiving much attention in the agricultural literature published in recent years. A study of melon (Cucumis melo L.) hybrid parents S ♂ and S ♀ and F 1 hybrids of K96-8×6-1, T-5×red1-28 and 94×8-1 was undertaken to determine if RP-HPLC could be adapted to the cultivar identification of melons. Chromatograms of the three inbred lines and F 1 hybrids of cultivars were shown to be different and were used to characterize those cultivars. RP-HPLC was shown to be a quick, replicable and reliable method of melon cultivar identification for general screening of seed lots.
出处
《作物学报》
CAS
CSCD
北大核心
2005年第2期177-182,共6页
Acta Agronomica Sinica
基金
国家自然科学基金(30470330)
国家自然科学基金(30470330)
浙江大学宁波理工学院青年博士基金(1050257G304)
新疆自治区科技厅自然基金项(200121107)资助
关键词
甜瓜
品种鉴定
反相高效液相色谱
Melon(Cucumis melo L.)
Cultivar identification
Reversed-Phase High Performance Liquid Chromatography
