摘要
目的:探讨无细胞短小棒状杆菌纳米级制剂(NCPP)杀灭病原微生物和抗肿瘤作用的机制。方法:采用纳米技术,制备NCPP,NCPP与短小棒状杆菌制剂(CPP)分别注射于小鼠腹腔,10d后,用酶联免疫吸附法(ELISA),检测肿瘤坏死因子(TNF-α)、干扰素(IFN-γ)、白细胞介素(IL-1β);用酶法、化学法检测一氧化氮(NO)、过氧化氢(H2O2)。结果:NCPP组与CPP组TNF-α、IL-1β、IFN-γ、H2O2、NO明显高于正常对照组(P<0.05)。结论:NCPP和CPP均能刺激巨噬细胞(Mφ)产生高水平的H2O2、NO、IL-1β、IFN-γ、TNF-α,但NCPP在增强IFN-γ的能力上明显高于CPP,而在产生TNF-α上明显低于CPP,提示NCPP的免疫调节作用高于CPP,而致炎症反应及纤维化低于CPP。
Objective: To study the mechanism of anti-pathogen and anti-tumor by nano scale corynebaterium parvum product (NCPP). Methods: NCPP were prepared by nanometer technology. Mice were injected NCPP and corynebacterium parvum product(CPP)into abdominal cavity; 10 day later,they were detected tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ)and interleukin-1β(IL-1β)secreted by celiac macrophages through enzyme linked immunosorbant assay(EILSA); they were detected nitrogen monoxide(NO) and hydrogen peroxide(H2O2),by enzymic and chemical method. Results: TNF-α,IFN-γ, IL-1β, H2O2 and NO secreted by mouse celiac macrophages,was obviously higher in NCPP and CPP groups than those in control group(P<0.05). Conclusion: Both NCPP and CPP can stimulate macrophages to produce high level of kill effect molecules of H2O2 and NO,and enhance the level of TNF-α,IFN-γand IL-1β secreted by celiac macrophages. NCPP can enhance the level of IFN-γ much more than CPP do,but produce less TNF-α than CPP do; this shows that NCPP is higher in immunoregulatory effect than CPP does,but lower in forming inflammation and fibrosis than CPP does.
出处
《山东大学学报（医学版）》
CAS
北大核心
2005年第1期30-33,共4页
Journal of Shandong University:Health Sciences
关键词
短小棒状杆菌
巨噬细胞
佐剂
免疫
纳米技术
Corynebacterium parvum
Macrophages
Adjuvants,immunologic
Nanotechnology
