摘要
目的 研究p6 3、Bcl 2和ki 6 7在宫颈上皮内瘤样病变 (CIN)、浸润癌中的表达及p6 3与Bcl 2、ki 6 7的不同点 ,探讨p6 3对宫颈病变发生的影响。方法 采用免疫组化PowerVisionTM二步法 ,检测 80例宫颈组织 (包括正常宫颈、宫颈炎、CIN、鳞癌及腺癌 )中p6 3、Bcl 2和ki 6 7的表达。结果 p6 3在正常宫颈上皮及鳞状化生上皮中主要表达在宫颈移行带区具有增生潜能的基底细胞 ;p6 3的阳性率与CIN分级呈正比 ,但CINⅢ与鳞癌阳性率差异无显著性 ;p6 3在腺癌中无表达。Bcl 2的表达与CIN分级无明显相关性。ki 6 7的表达与宫颈癌组织分化程度成正相关。Bcl 2和ki 6 7在鳞癌、腺癌中阳性表达率均无差异。结论 p6 3在宫颈癌形成早期可能起促癌作用 ,且可能是鳞状上皮源性肿瘤的标记物。p6
Objective To investigate the effect of p63 protein in the cervical carcinoma through the expressions and the difference of p63,Bcl-2 and ki-67 in the cervical intraepithelial neoplasia (CIN) and invasive carcinoma.Methods Immunohistochemical techniques(Power VisionTM) were used to study the expressions of p63,Bcl-2 and ki-67 in 80 cases of cervical epithelial (normal or reactive epithelial changes,CIN,squamous cell carcinoma and adenocarcinoma ).Results p63 expression by immunostaining delineated basal cells with high proliferative potentiality of the cervical transformation zone.In CIN,p63 expression was direct correlated with grades of CIN,but the difference between CINⅢ and squamous cell carcinoma was no significant.The expression rate in adenocarcinoma was 0%.In CIN,Bcl-2 expression was not related to the grades of CIN.The expression rate of Bcl-2 was more limited than that of p63.The expression rate of ki-67 was related to the histological differentiation degree.No significant difference of Bcl-2 and ki-67 positive expressions were detected in squamous cell carcinoma and adenocarcinoma.Conclusion p63 may promote transformation of primary cervical carcinoma.p63 may be a marker of tumor of squamous epithelia origin.p63 is linked to cell proliferation.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2005年第1期52-54,57,共4页
Journal of Harbin Medical University
关键词
基因表达
宫颈上皮内瘤样病变
宫颈肿瘤
gene expression
cervical intraepithelial neoplasia
cervical carcinoma
