摘要
利用ATPase定位技术,对水稻品种(OryzasativaL.cv.Minghui63)胚乳细胞发育中后期淀粉体和蛋白体的ATPase活性进行了超微细胞化学定位。结果表明,在淀粉体内外膜上、淀粉粒间的通道上和淀粉体四周的无定形物上呈现显著的ATPase活性。蛋白体Ⅰ和蛋白体Ⅱ的膜上和四周的囊泡、小泡上均出现ATPase活性产物。另外,胚乳细胞的胞壁和质膜,糊粉层和亚糊粉层细胞的胞壁、质膜、细胞核和胞间连丝上也有定位的ATPase活性产物分布。根据ATPase活性产物分布特点,推测淀粉体内的网状通道是便于养分进入淀粉体内部的转运通道。淀粉体膜和蛋白体膜上的ATPase主要是为养分进入内部提供跨膜动力。
The ultracytochemical localizations of ATPase activity on amyloplasts and protein bodies were studied by using a lead precipitation technique at middle and late developmental stage of endosperm in rice(Oryza sativa L. cv. Minghui 63). The results showed that the inner and outer plasma membrane of amyloplasts, the web-like passages among starch grains and the amorphous substance surrounding the amyloplasts exhibited high ATPase activity. ATPase activity products were present on the membranes of protein body Ⅰ and protein body Ⅱ, and on the vac- uoles and vesicle-like structures surrounding protein bodies. Active products of ATPase were also located on the plasma membrane and cell wall of starchy endosperm cells, on the cell wall, plas- ma membrane, nucleus and plasmodesma of aleurone and subaleurone layer cells. According to the distribution pattern of active products of ATPase, we infer that the web-like passage in amyloplast may be the transporting channel for nutrients flowing into the amyloplast. The ATPase on amylo- plasts and protein bodies can supply the power for nutrients passing through the plasma mem- branes.
出处
《实验生物学报》
CSCD
北大核心
2005年第1期7-15,共9页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金(30070468)中国博士后科学基金(2003033468)
