摘要
目的 :观察高同型半胱氨酸血症对血管内皮功能的影响。方法 :建立兔高同型半胱氨酸血症模型。将 18只新西兰兔随机分为 :正常对照组 (control组 ) 6只、高蛋氨酸饮食组 (M组 ) 12只 ;于实验第 4周始 ,将M组再随机分为两组 ,M +0组 6只 ,继续饲高蛋氨酸饮食 ;M +F组 6只 ,在高蛋氨酸饮食基础上 ,再加以叶酸、VitB12 ,继续观察 3周 ;6周时统一处死动物 ,取腹主动脉 ,制备主动脉血管环 ,比较M +0组与M +F组及C组主动脉血管对Ach的最大舒张反应。同时 ,对 3组高同型半胱氨酸血症形成过程中 0周、3周、6周时血清中Hcy、ET - 1、Ang -II、NO2 ˉ/NO3 ˉ、NOS各指标及处死动物时局部血管组织中ET - 1、Ang -II、NO2 ˉ/NO3 ˉ、NOS指标进行检测并进行比较。结果 :(1)M +0组主动脉血管对Ach的最大舒张反应性 (Emax=2 6 . 73± 4 . 5 1)低于M +F组 (Emax=4 7. 84± 5 . 6 2 ,P <0 . 0 5 )及control组 (Emax=5 6 . 4 2± 7 .82 ,P <0 . 0 5 ) ;(2 ) 3周时 ,M +0组及M +F组血清中Hcy、ET - 1、Ang -II各指标均明显高于对照组及 0周时 (P <0 . 0 5 ) ;NO2 ˉ/NO3 ˉ、NOS明显低于对照组及 0周时 (P <0 . 0 5 ) ;(3) 6周时 ,M +0组上述指标继续升高 ;M +F组血清中Hcy低于M +0组 (P <0 . 0 5 ) ;NO2 -/NO3 -。
AIM: To investigate the effect of hyperhomo cysteine on endothelial cell function. METHODS: By establishing hyperhomocysteinemia model, 18 male New Zealand rabbits were divided into control group (control group) and high-methi o nine-diet group (M group). At the end of 3 weeks, M group was divided again into M+0 group (continuing high methionine-diet) and M+F group (high-methionine-diet plus folic acid, vitamin B 12). At the end of 6 weeks, isolated aortic rin g s were made and the maximum vasodilation of the aortic rings to Ach was investig ated. Meanwhile, the plasma concentrations of Hcy, NO, ET-1, Ang II at 0 week, 3 weeks and 6 weeks and the contents of NO 2-/NO 3-, Ang Ⅱ, ET-1, NOS i n regional vascular tissue at 8 weeks were also measured. RESULTS: (1) In contrast to M+F group and control group, the max imum vasodilation to Ach were decreased (E max=26.73±4.51 vs 47.84 ±5.62, 56.42±7.82, P<0.05) in M+0 group. (2) At the end of 3 weeks, th e plasm a concentration of Hcy, Ang Ⅱ, ET-1 were increased in M group compared with C g r oup and at 0 week (P<0.05), NO and NOS were decreased (P<0.05). (3) At the end of 6 weeks, the plasma concentration of Hcy, Ang Ⅱ, ET-1 were conti nu ally increased in M+0 group. In M+F group, by folic acid and vitamin B 12 t reatment, the level of Hcy was decreased compared with M+0 group (P<0.05). The levels of NO and NOS were increased (P<0.05). However, ET-1, Ang Ⅱ we re not obviously changed. CONCLUSIONS: Hyperhomocysteinemia obviously restrains the endoth elial vasodilation. The effect is initiated by changing the tissue regional leve l of ET-1, Ang Ⅱ and NO. By early folic acid and vitamin B 12 treatmen t, the endothelial cell function may be protected.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第2期339-341,共3页
Chinese Journal of Pathophysiology
基金
山西省留学归国人员基金资助资助项目 (No .2 0 0 10 2 9)
