摘要
目的 探讨血管性痴呆 (VD)患者与其血浆同型半胱氨酸水平的关系。方法 用高效液相色谱仪和电化学检测法测定 1998年 2月至 2 0 0 0年 2月收治的 37例VD患者的血浆总同型半胱氨酸水平 ,并与 40名正常同龄对照组及 40例非痴呆脑梗死组比较。运用多聚酶链反应 限制性内切酶片段长度多态性技术 (PCR RFLP)检测N5,N10 亚甲基四氢叶酸还原酶 (MTHFR)基因多态性 ,同时测定血浆叶酸及维生素B12 水平。结果 VD患者血浆总同型半胱氨酸水平显著高于正常同龄对照组和非痴呆脑梗死组 ;MTHFR基因型有 3种 ,即纯合子 (T/T)型、杂合子 (T/C)型和纯合子 (C/C)型。 3组基因型和等位基因频率相比 ,差异均无显著性 ;VD组血浆叶酸及维生素B12 水平明显低于正常同龄对照组和非痴呆脑梗死组 (P <0 .0 5 )。结论 高同型半胱氨酸血症可能是VD发病的一个新的危险因素。
Objective To study the relationship between vascular dementia and plasma homocysteine(Hcy) levels.Methods 37 VD patients,40 normal aged subjects and 40 patients with nondemented cerebral infarction were recruited in the study.Their plasma total Hcy levels were measured using high-performance liquid chromatography with electrochemical detection.The polymorphisms of N 5,N 10 -methylenete-trahydrofolate reductase(MTHFR),gene were analyzed using PCR-RFLP.Plasma folate,serum vitamin B 12 were determined for all of them.Results Mean total plasma homocysteine concentrations were significantly higher in VD patients than in the normal elderly subjects(P<0.001) and patients with nondemented cerebral infarction(P<0.05).There were three kinds of MTHFR genotype: T/T (homozygous mutation),T/C (heterozygous mutation) and C/C (wild-type).There was no significant difference between the frequencies of the genotypes and alleles of MTHFR gene in three groups (P>0.05).Plasma folate and serum vitamin B 12 concentrations were significantly lower in AD group than in control group( P<0.05) and patients with nondemented cerebral infarction(P<0.05).Conclusion Hyperhomocysteinemia may be a new risk factor for VD.
出处
《中华老年心脑血管病杂志》
CAS
2000年第5期318-321,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
