摘要
研究了菌体生长、产物合成及底物消耗的最佳关系,通过5L实验罐的正交试验,确定了以兽疫链球菌NUF—036分批发酵生产透明质酸的工艺条件:通风量2.0L/min,搅拌转速600r/min,pH=7.5,温度35℃.在此条件下,透明质酸的产量为3.7g/L,分子质量为3.8×106Da.建立了兽疫链球菌NUF—036分批发酵生产透明质酸的动力学模型.
Optimum relationship of substance consumption, biomass growth and products synthesizes was studied in this paper. The optimum batch fermentation condition in (5 L) fermentor was that the aerobic rate, 2.0 L/min, the agitation rate, 600 r/min, pH =7.5, temperature, 35 ℃ through orthogonal design, in which the molecular weight of hyaluronic acid (HA) was 3.8×10~6Da,and HA concentration increased to 3.7 g/L. The model of Streptococcus zooepidemicus NUF—036 in batch fermentation was established in this paper.
出处
《宁夏大学学报（自然科学版）》
CAS
2004年第4期368-373,共6页
Journal of Ningxia University(Natural Science Edition)
基金
宁夏自然科学基金资助项目(A2001)
