摘要
目的观察17-α雌二醇对体外培养成骨细胞增殖和成骨功能的影响。方法 用植块培养法从乳鼠中分离出成骨细胞,倒置显微镜观察其形态,碱性磷酸酶(ALP)染色来鉴定细胞。然后用不同浓度的17-α雌二醇作用于成骨细胞,使用四甲基偶氮唑蓝(MTT)比色分析、ALP含量测定、细胞钙含量测定来检测其对成骨细胞增殖和成骨功能的影响。结果 17-α雌二醇以剂量方式促进各项指标的表达,促进成骨细胞的增殖和骨形成。结论17-α雌二醇具有促进成骨细胞的增殖和骨形成作用,值得作进一步的研究。
Objective To investigate the effect of 17-a estradiol on osteoblasts (OB) cultured in vitro. Methods OB were isolated from rats and the cell morphology was observed by alkaline phosphtase (ALP) staining. 17-a estradiol in different concentrations was added to the OB, which were incubated. The effect of 17-a estradiol on the proliferation and osteogenesis of OB was monitored by MTT analysis, ALP activity and calcium contents determination. Results The MTT(OD), ALP and calcium contents of the cultured cells as well as the cell proliferation were higher in 17-a estradiol group than those in control group( P < 0.05) in a dose-dependent manner. It suggested that 17-a estradiol promoted the proliferation and osteogenesis of OB. Conclusions 17-a estradiol promotes the proliferation of OB and bone formation.
出处
《中国骨质疏松杂志》
CAS
CSCD
2004年第4期435-438,共4页
Chinese Journal of Osteoporosis
