摘要
甘薯叶片愈伤组织生长受 ^(60)Co γ-射线的影响很显著。经100Gy 剂量照射的愈伤组织的重量仅为对照的一半左右;蛋白质含量都低于对照。在试验期间蛋白质含量的增加出现2个高峰。愈伤组织经辐照后,脂质过氧化作用产物 MDA 含量比对照高,尤以高剂量(100Gy)辐照的增加更高。辐照后4周,MDA 含量开始下降,可降到低于对照的水平,10周后又上升到试验期间的最高水平。在试验开始后6周内,无论辐照与否,愈伤组织中脂氧合酶的活性都处于较低的水平,6周后其活性显著增加。试验期间愈伤组织中过氧化氢酶活性出现一个高峰。经高剂量(30Gy 和100Gy)照射的,其高峰出现期要比5Gy 处理和对照组早2周。无论辐照组和对照组,过氧化物酶在试验期间都出现2个活性高峰。100Gy 照射的酶活性峰值比对照组高,其它2个照射剂量的高峰值比对照组低。
The growth of sweet potato leaf callus was affected remarkably by ^(60)Co γ-ray irra- diation.The weight of callus irradiated at 100 Gy was only about the half of the CK,the protein content was lower than that in the CK.Two peaks of protein content appeared during the experi- ment.The production of the lipid peroxidation product (MDA) of the irradiated callus was higher than that of the CK,especially when irradiated at 100 Gy,four weeks after irradiation,the MDA content began to fall down and remained at lower level for about four weeks.Ten weeks after,the MDA content began to go up again and reached the highest level in the period of experiment.Dur- ing the six weeks after the starting of the experiment,in the lipoxygenase activity both irradiated and unirradiated callus was at lower level.Six weeks after,lipoxygenase activity increased remark- ably.The catalase activity peak in callus appeared only once during the experiment.The catalase activity peak in callus irradiated at high dose(30 and 100 Gy)appeared about two weeks earlier than that in callus irradiated at 5 Gy and that in the CK.And two peroxidase activity peaks in both the unirradiated and irradiated calli appeared during the experiment.The peak value of the enzyme activity in callus irradiated at 100 Gy was higher than that in CK.The peak value in the other two treatments with calli irradiated at 5 and 30 Gy was lower than in the CK.The results described above were discussed.
出处
《上海农业学报》
CSCD
1993年第4期45-52,共8页
Acta Agriculturae Shanghai
基金
联合国国际原子能机构研究合同 No.5825/RI/RB 提供部分资助~~
关键词
甘薯
愈伤组织
^60Coγ-射线
辐照
Sweet potato
Callus
^(60)Co γ-ray irradiation
Catalase
MDA(malonaldehyde)
Peroxidase
Lipoxygenase
Enzyme activity
