摘要
目的探讨纳络酮对脂多糖诱导原代培养星形胶质细胞释放谷氨酸的抑制效应。方法体外培养星形胶质细胞于融合状态,随机分为5组(1)对照组(L0+N0);(2)1μg·ml-1脂多糖组(L1+N0);(3)1μg·ml-1脂多糖+0.5μmol·L-1纳络酮组(L1+N0.5);(4)1μg·ml-1脂多糖+1.0μmol·L-1纳络酮组(L1+N1.0);(5)1μg·ml-1脂多糖+2.0μmol·L-1纳络酮组(L1+N2.0)。各组培养液均换成Neurobasal/B27无血清培养液后,在相应组中加入上述相应终浓度的脂多糖和纳络酮,继续培养2h。用反相高效液相色谱分析方法测定各组细胞外液中谷氨酸含量。结果L1+N0组中谷氨酸含量明显高于L0+N0组,其差异有极显著性意义(P<0.05);L1+N0.5、L1+N1.0、L1+N2.0组内谷氨酸的含量则随着纳络酮用量的增加而逐渐降低,其中L1+N2.0组与L1+N0组相比,谷氨酸含量差异有显著性(P<0.05)。结论纳络酮能抑制脂多糖刺激大脑皮质星形胶质细胞释放谷氨酸,具有一定的脑保护作用。
Objective To detect effects of naloxone in lipopolysaccharide-induced glutamate release of primary rat neonatal astrocytes.Methods Astrocytes cultured in vitro were randomly divided in 5 groups.(1)control(L_0+N_0);(2)1μg·ml^-1 lipopolysaccharide(L_1+N_0);(3)1μg·ml^-1 lipopolysaccharide and 0.5μmol·L^-1 naloxone(L_1+N_~0.5 );(4)1μg·ml^-1 lipopolysaccharide and 1.0μmol·L^-1 naloxone(L_1+N_~1.0 );(5)1μg·ml^-1 lipopolysaccharide and 2μmol·L^-1 naloxone(L_1+N_~2.0 ).Glutamate of extracellular fluid were detected by reversed-phase HPLC.~Results It was a significant difference between the group of L_1+N_~1.0 and the group of L_1+N_0 P<0.05.The more naloxone was added in culture, the less was glutamate of extracellular fluid.It was a significant difference between the group of L_1+N_~2.0 and the group of L_1+N_0,in glutamate of extracellular fluid P<0.05.Conclusion Naloxone could decrease the reseasing of glutamate in astrocytes of cerebral cortex,and it can protect the brain.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2004年第6期515-517,共3页
Journal of Apoplexy and Nervous Diseases
