摘要
本研究通过热激(43～45℃)和2种外源激素吲哚乙酸(IAA)、乙烯利对6个大豆品种的花荚期植株进行单一处理和复合处理,研究不同处理对大豆HSP70基因表达的影响.Northern杂交结果显示,单一热激处理时所有供试品种的HSP70基因表达量明显高于常温(30～32℃)处理组.用激素(IAA或乙烯利)单一处理时,所有供试品种的HSP70基因表达量与各自的水处理对照组无明显差异,当热激附加IAA(0.05～0.15g/L)复合处理时,所有6个品种的HSP70 mRNA转录量均高于各自的单一激素处理,而热激附加乙烯利(1～7ml/L)复合处理的作用效果较为复杂,HSP70基因的表达水平因品种而异.实验结果说明,热激处理能够提高大豆植株花荚离层组织HSP70基因的表达水平,外源激素(IAA或乙烯利)单独处理基本不能促进大豆HSP70基因的表达,但IAA处理能够加强热激对该基因的表达作用,表现协同效应,这种协同效应对于提高大豆抗逆性和降低花荚脱落率具有实际意义.
The objectives of this experiment are to investigate effects of treatments of heat shock (HS, 43~45℃) or exogenous hormones (IAA, ethephon) only, and HS plus each of two hormones on expression levels of HSP70 gene from the abscission layer tissues of plant flowers and pods of six soybean cultivars during the early flowering period by Northern blotting analysis. The experimental results showed that HSP70 mRNA accumulations from all six varieties treated by HS were significantly higher than those of normal temperature(30~32℃) treatments, which indicated that HS could enhance the expression levels of HSP70 gene from different soybean genotypes. There weren't notable differences between expression levels of HSP70 gene from all six cultivars treated each of two hormones only and those done with water (ck), indicating the treatments of exogenous hormones (IAA or ethephon) couldn't almost enhance this gene expression. The effects of HS plus ethephon(1~7ml/L)treatment on HSP70 gene expression in soybean were relatively complex, that is, the expression levels of HSP70 gene from various soybean cultivars treated by HS plus ethephon were different. However, mRNA accumulations of HSP70 in all six cultivars treated by HS plus IAA were higher than those done by IAA only, demonstrating that IAA could promote the effect of HS treatment on HSP70 gene expression in soybean, and it seem to prove that there were synergetic effect between HS plus IAA treatment on the HSP70 gene expression in soybean, and this effects would be important for increasing tolerant stress such as heat and decreasing dropping rate of flower-pod of soybean plants.
出处
《分子植物育种》
CAS
CSCD
2005年第1期21-25,共5页
Molecular Plant Breeding
基金
国家自然科学基金课题(30270836)
科技攻关计划大豆种质创新课题的资助
国家863、973项目的部分资助。
