摘要
目的 :探讨微卫星标志物D3S12 5 5 ,C13 1169,IFNA ,D9S171改变及p16的异常甲基化与肺癌发生发展的关系 ,以及其在肺癌早期诊断和转移判断方面的价值。方法 :应用聚合酶链反应 (PCR)结合微卫星银染分析方法检测肺癌组织微卫星标志物的改变。结果 :5 6例肺癌组织标本中 3p上微卫星改变 2 6例 (4 6% ) ,9p上有微卫星改变的2 3例 (4 1% ) ,17p上有微卫星改变 15例 (2 6 8% )。 3p、9p和 17p上微卫星改变的总检出率 69.6% (3 9/ 5 6)。 44 .6%(2 5 / 5 6)的肺癌组织标本检出p16基因异常甲基化。 5 6例肺癌标本中 ,43 (76.8% )例至少检出一个以上的微卫星改变或p16基因异常甲基化。 2 3例肺部良性疾病的组织标本中 3p、9p和 17p上均没有发现微卫星标志物的改变及p16基因异常甲基化。结论 :3p ,9p和 17p上微卫星改变和p16基因异常甲基化是肺癌发生、发展过程中较早出现的遗传性改变之一 ,且与肺癌转移密切相关 ,有望应用于肺癌的早期诊断和转移判断。
Objective: To investigate the relationship between alternations of microsatellite markers and p16 gene methylation, and the genesis metastasis of lung cancer. Methods:Five microsatellite markers D3S1255, CI3-1169, IFNA, D9S171 and p16 gene methylation were analysed in 56 cases of lung cancer by polymerase chain reaction based silver staining assay for microsatellite markers and Methylation.The microsatellite markers were analyzed in lung cancer by poly merase chain reaction based silver staining assay.Results:39 (69.6%) out of 56 cases of lung cancer showed microsatellite alternations and 25(44.6%) out of 56 cases of lung cancer showed p16 gene methylation. None of benign lung tissues show this alternations. There was correlation between the microsatellite alternations on 3p and differentiation stage, and there was correlation between the microsatellite alternations on 9p, 17p and p16 gene methylation , and lymphnode metastasis. Conclusions:Microsatellite alternations and p16 gene methylation in lung cancer appear to be the common and specific event, and they may be valuable molecular markers for determination of early diagnosis and metastasis of lung cancer.
出处
《河南医学研究》
CAS
2004年第4期289-292,共4页
Henan Medical Research
基金
河南省科技攻关计划项目 (9840 2 44 0 0 )
