摘要
用4株小鼠肝炎病毒(MHV1,MHV3,A59,JHM)分别感染DBT细胞,收获病毒,提取病毒RNA。根据病毒基因的特异性保守序列设计引物,在最佳条件下进行RT-PCR,建立了RT-PCR检测方法,即两步法和一步法,分别扩增出600bp,375bp的特异性核酸片段,其中,一步法检测MHV更快速、简便。
Four different strains of mouse hepatitis virus (MHV), MHV 1, MHV 3, A 59 and JHM, were used to infect DBT cells. Viral RNA was extracted from the culture liquid. Two pairs of primers were designed according to the specific conservative gene sequence of MHV. RT-PCR was performed under the optimum conditions. Two RT-PCR procedures were established for MHV detection: one-step and two-step procedures, which amplified specific nucleic acid segments of 600 bp and 375 bp, respectively. Comparatively, the one-step RT-PCR was quicker, simpler and more convenient.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2004年第6期762-763,772,共3页
Journal of Southwest Agricultural University
基金
重庆市科委资助项目(99-5470)
