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DNMT1靶向RNAi重组体对胃癌细胞周期的影响

RNA interfering on the cell cycle of gastric cancer by the recombinantplasmid affecting DNMT1 gene expression
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摘要 目的 :本研究利用RNA干扰 (RNAinterfering ,RNAi)技术 ,以DNMT1(DNAmethyltransferase 1)为靶基因 ,设计构建重组体 ,研究其对胃癌细胞周期的影响。方法 :设计有小发夹结构的两条DNA序列 ,经退火成互补双链 ,再克隆至载体pTZU6 +1中构建重组体 ,经鉴定正确后 ,转染胃癌细胞AGS ,最后使用流式细胞仪分析细胞周期。结果 :转染后的胃癌细胞的S期细胞较前有明显减少 ,G2 -M期细胞明显增多。结论 :DNMT1靶向RNA干扰重组体转染到胃癌细胞中后促使S期细胞进入G2 -M期 ,最后衰老死亡。从而为肿瘤的治疗开辟了新途径。 Objective:To clone the recombinant plasmid affecting gene DNMT1 expression by RNA interfering and transfect it into AGS in order to further search its effects on the cell cycle of gastric cancer AGS.Methods:Two DNA sequences containing short hairpin structure was designed and synthesized.The complement form was obtained by annealing and inserted into vector pTZU6+1.The recombinant plasmid was transfected into AGS.Finally the cell cycle was evaluated through flow cytometer.Results:The S cycle cells of the transfected AGS dropped dramatically.And the G2-M cycle cells increased significantly.Conclusion:Successful cloning and transfecting the recombinant make it possible to search new gene therapy for the tumors.
作者 陈道荣 王丕龙 黄爱龙
机构地区 重庆医科大学临床学院消化内科 重庆医科大学病毒性肝炎研究所
出处 《重庆医科大学学报》 CAS CSCD 2004年第6期738-741,共4页 Journal of Chongqing Medical University
关键词 DNMT1 RNA干扰 胃癌细胞 细胞周期 DNMT1 RNA interfering Gastric cancer Cell cycle
分类号 R734 [医药卫生—肿瘤]
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参考文献12

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二级参考文献6

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重庆医科大学学报
2004年 第6期

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