摘要
目的本实验通过维甲酸对乳腺癌细胞的钠碘转运体(NIS)基因及蛋白表达和摄碘功能的影响,探讨乳腺癌细胞NIS的表达特点和调控因素。方法我们采用乳腺癌细胞MDA-MB453进行培养,分别给予不同浓度的维甲酸(RA)进行刺激72h。采用RT-PCR方法检测细胞中NIS的mRNA表达;用WesternBloot方法检测NIS蛋白水平;采用125I测定乳腺癌细胞摄碘功能的变化。结果乳腺癌细胞MDA-MB453在经过RA刺激后,细胞中NIS的mRNA表达比对照组有明显增加,并且与RA浓度成正比。细胞中NIS的蛋白表达比对照组也有明显增加,并且与RA浓度相关。经过RA刺激的乳腺癌细胞摄碘能力明显增加。结论维甲酸对乳腺癌细胞MDA-MB453的NIS基因和蛋白表达均有促进作用,而且促进乳腺癌细胞的摄碘,这提示维甲酸可能通过调节乳腺癌NIS基因和蛋白的表达,从而影响乳腺癌细胞对放射性碘治疗的敏感性。
Objective:To investigate the effect of retionic acid on gene and protein expression of sodium-iodide symporter(NIS)and on the activity of iodide uptake in breast cancer cell(MDA-MB453).Methods:The breast can-cer cell MDA-MB453was cultured with negative control culture or with different concentration of retinoic acid for72hours.NIS gene mRNA in breast cancer cells was determined by reverse transcriptase-polymerase chain reaction(RT-PCR).NIS protein was checked by Western Blot.And the activity of iodide uptake was also assessed.Results:Gene mRNA and protein of sodium iodide symporter could be found increasing along with the concentration of retinoic acid.And augment of the activity of iodide uptake had also been indetified.Conclusion:Retionoic acid may play a role in iodide-uptake modulating in breast cancer cells by the effect on NIS gene and protein expression.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第21期11-14,共4页
China Journal of Modern Medicine
基金
Keysubjectfundof"135"projectinJiangsuHealthyOffice.ProjectNumber:2001-3111
