摘要
目的 :研究外源性牛血浆纤维粘连蛋白对体外培养的大鼠成骨细胞增殖分化的影响。方法 :采用体外培养技术原代培养大鼠成骨细胞 ,用AlamarBlue摄入法检测不同浓度的牛血浆纤维粘连蛋白对大鼠成骨细胞增殖的影响 ,ELISA方法测定碱性磷酸酶活性的改变。结果 :AlamarBlue摄入法结果显示牛血浆纤维粘连蛋白能够促进大鼠成骨细胞的增殖 ,当浓度为 40 μg/ml时 ,与对照组相比有显著性差异 (P <0 .0 5 ) ,当浓度为 5 0μg/ml时作用差异极显著 (P <0 .0 1) ,ELISA方法结果显示牛血浆纤维粘连蛋白能够增加大鼠成骨细胞碱性磷酸酶活性 ,各组与对照组相比均有显著性差异 (P <0 .0 5 ) ,并有明显的剂量依赖性。结论
Objective: To investigate the effects of bovine plasma fibronectin on the proliferation and differentiation of rat osteoblasts. Methods:Rat osteoblasts were cultured and identified. AlamarBlue were used to determine the effect of various concentration of bovine plasma fibronectin on the proliferation of the osteoblasts. ELISA method was used to determine the alkaline phosphatase activity of the cells. Results: Fibronectin stimulated the proliferation of rat osteoblasts at 40 μg/ml (P<0.05) or 50 μg/ml (P< 0.01).Fibronectin increased the alkaline phosphatase activity of the cells at 10-50 μg/ml dose-dependently (P<0.01) .Conclusions: Certain concentration of bovine plasma fibronectin can stimulate the proliferation and differentiation of rat osteoblasts in vitro.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2004年第6期730-732,共3页
Journal of Practical Stomatology
