摘要
在显性细胞核雄性不育系Rs10 4 6A和双低油菜品种Samourai构建的回交分离群体中 ,运用AFLP和SSR两种标记技术构建了一个甘蓝型油菜 (BrassicanapusL .)的分子标记遗传连锁图谱。该图谱共包含 138个AFLP标记、83个SSR标记和 1个形态标记 ,分布于 18个主要连锁群、2个三联体和 1个连锁对上 ,图谱总长度为 2 6 4 6cM ,偏分离标记的比例为 11 7%。显性细胞核雄性不育基因Ms被定位到第 10连锁群 (LG10 )上。同时 ,偏分离标记聚集于第 8连锁群 (LG8)和第 16连锁群 (LG16 )的末端 ,形成了十分明显的偏分离标记密集区域。研究结果对于油菜核不育两型系的分子标记辅助选择育种具有重要意义 。
A backcross population derived from a cross between the dominant genic male sterile line Rs1046A and the double-low cultivar ‘Samourai’ was used for linkage map construction in Brassica napus.A linkage map with a total length of 2 646 cM was developed using 138 AFLP markers,83 SSR markers and one morphology trait,which were distributed over 18 major linkage groups,two triplets and one linkage pair.11.7% of the mapped markers distorted from the expected 1∶1 ratio.The dominant genic male sterility gene (Ms) was mapped in LG10 and surrounded by five closely linked AFLP markers.Meanwhile,two evident marker segregation distortion regions were observed in both LG8 and LG16.The map constructed in the present study and the mapping of Ms gene are highly valuable in designing marker-assisted breeding program for the genic male sterile two-type line in Brassica napus.They are also important to map-based cloning of the Ms gene and to better understanding of the mechanism of genic male sterility at molecular level.
基金
国家重点基础研究发展规划项目 (编号 :2 0 0 1C13 10 880 7)
国家自然科学基金 (编号 :3 0 0 80 0 12 )
湖北省自然科学基金 (编号 :2 0 0 0J110 )
教育部博士点基金资助~~
