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血清蛋白质组学研究中白蛋白和IgG去除方法的建立与评价 被引量：11

Establishment and evaluation of the method for removing albumin and IgG in serum proteomics
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摘要 目的建立和评价血清蛋白质组学研究中白蛋白和IgG的去除方法。方法应用Micro Bio-Spin column过滤纯化血清蛋白,去除白蛋白和IgG,利用双向凝胶电泳(2-DE)分析评价血清蛋白过滤效果。结果建立了血清白蛋白和IgG去除方法;过滤后大部分血清白蛋白和IgG能够去除,2-DE图谱中低丰度蛋白质分辨率增加。结论本研究建立的血清蛋白质纯化技术,可有效应用于疾病的血清蛋白质组学研究。 s: Objective To establish and evaluate the method for removing albumin and IgG in serum proteomics. Methods Elimination of serum albumin and IgG was achieved by filtering with Micro Bio-Spin column and the efficiency of purification evaluated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Results The method for removing serum albumin and IgG was established, which enabled visualization of low-abundance proteins better in 2-DE map. Conclusion The established method for purifying serum proteins can be effectively applied in serum proteomic study of diseases.
作者 李雪华 李欣 徐守军 丁彦青
机构地区 南方医科大学南方医院病理科 南方医科大学肿瘤研究所 军事医学科学院
出处 《第一军医大学学报》 CSCD 北大核心 2004年第11期1233-1235,共3页 Journal of First Military Medical University
基金 广东省科技重大专项(2003A308401) 国家重点基础研究发展规划项目(973)(2001CB510208)~~
关键词 蛋白质组学 白蛋白 IGG 去除方法 双向凝胶电泳 蛋白质纯化技术 serum proteomics two-dimensional polyacrylamide gel electrophoresis albumin IgG
分类号 R341 [医药卫生—基础医学]
  • 相关文献

参考文献15

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共引文献9

同被引文献80

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  • 2丁娜,李树花.支气管肺泡灌洗液肿瘤标志物检测对肺癌诊断的价值[J].天津医药,2007,35(1):30-32. 被引量：8
  • 3刘希成,梁恒,田真,张霖,陈阳.血清蛋白质组样品处理方法的比较分析[J].第四军医大学学报,2007,28(7):640-640. 被引量：2
  • 4Zalesska-Krecicka M, Krecicki T. Nasopharyngeal carcinoma coexistent with lymphonodular tuberculosis, difficulty with diagnosticscase raport [J]. Otolaryngol Pol, 2005,59(4):607-609.
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引证文献11

二级引证文献21

第一军医大学学报
2004年 第11期

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