摘要
采用高效毛细管电泳法测定人尿中姜黄素的含量,建立了一种测定体液中姜黄素的简便的分析方法。所用缓冲溶液为15mmol/L的硼砂溶液(pH9 24),运行电压为20kV,检测波长262nm。姜黄素的峰面积与其质量浓度的线性关系良好,其线性范围为10～300mg/L,健康人尿中姜黄素的4个浓度水平的添加回收率均大于96 3%,相对标准偏差(RSD)小于2 3%。该法灵敏、快速、准确,可用于体液中姜黄素的批量检测。
The major component of the plant curcuma longa (a widely cultivated tropical plant in Asia and Central America) is curcumin. Curcumin has been reported to have very strong anti-inflammatory, anti-carcinogenic, anti-oxidant, antiallergic, anti-bacterial, and (anti-tumor) activities. Little is known about the absorption, distribution, and metabolism of curcumin in human beings. The first step in in vivo physiological and pharmacokinetic studies is to develop a method to measure curcumin in body fluid. A rapid capillary electrophoretic (CE) method with diode array detection was established for the determination of curcumin in human urine. It could be rapidly determined within 2.5 min. The optimized experimental conditions were as follows: 15 mmol/L Na_2B_4O_7 as buffer, applied voltage 20 kV, temperature 25 ℃ and detection wavelength 262 nm. The method has been validated and shows good performance with respect to selectivity, reproducibility and limit of detection. Curcumin had good linearity in the range of 10-300 mg/L, and the recoveries of curcumin added in urine were more than 96.3% with relative standard deviations (RSDs) less than 2.3%. The method is sensitive, fast and accurate and can be used to determine curcumin in urine.
出处
《色谱》
CAS
CSCD
北大核心
2004年第6期609-612,共4页
Chinese Journal of Chromatography
基金
国家自然科学基金资助项目(No.90209048)
中国科学院前沿领域创新基金资助项目(No.K2002A11)
中国科学院重要方向性项目(No.KGCX2 SW 213).
关键词
毛细管电泳法
姜黄素
尿
capillary electrophoresis
curcumin
urine
