摘要
目的 :研究拉米夫定对慢性乙型肝炎 (慢乙肝 )患者血清及PBMC内HBVDNA的抑制作用。方法 :应用荧光定量PCR方法动态观察 72例拉米夫定治疗组和对照组患者血清及PBMC内HBVDNA的含量变化。结果 :治疗组血清HBVDNA在治疗 6周时 ( 2～ 8周 ) 2 1例转阴 ,PBMC中HBVDNA在治疗 16周 ( 8～ 2 4周 )时 17例转阴。结论 :拉米夫定对慢乙肝患者血清HBVDNA有较强的抑制作用 ,起效快 ,同时 ,随着治疗时间的延长 ,对PBMC内HBVDNA也有一定的抑制作用 。
Objective:To study the dynamic changes of hepatitis B virus DNA in serum and in peripheral mononuclear cells in the patients with Lamivudine therapy.Methods:Seventy-two patients with chrnic hepatitis B virus (HBV) infection were investigated for HBV DNA both in serum and in peripheral blood mononuclear cells(PBMCs) by fluorescence quantitative polymerase chair reation(PCR),during and after Lamivudine treatment.Results:HBV DNA both in serum and in PBMCs, become negative in 21 cases and 17 cases respectively during Lamivudine treatment. The average conversion period of HBVDNA was 6w (2-8w) in serum and 16w (8-24w) in PBMCs. Conclusion:Lamivudine has remarked inhibitory effect on HBV DNA both in serum and in PBMCs. The inhibitoty effect on HBV DNA in PBMCs was sloowe than in serum.
出处
《中西医结合肝病杂志》
CAS
2004年第4期197-199,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
武汉大学创新基金资助(No:301270054)
