摘要
目的 了解饮用水雌激素污染物污染状况。方法 应用气相色谱 /质谱 (GC/MS)分析技术、人乳腺癌(MCF/ 7)细胞增殖试验和子宫湿重实验 ,对某市饮用水雌激素污染物进行检测。结果 GC/MS分析表明 ,水源水中有机污染物 10 0余种 ,出厂水中 80余种 ;其中作为可疑雌激素物质的邻苯二甲酸二 ( 2 甲基丙基 )酯、邻苯二甲酸二丁酯检出率分别为 10 0 %和 90 %。此外 ,还检出 2 ,4 二甲基酚等酚类物质。嘉陵江水源污染重于长江 ,出厂水均为丰水期污染重于枯水期 ,而以C厂和E厂出厂水水质较好。生物学评价结果显示 ,水源水、出厂水有机提取物均刺激MCF/ 7细胞增殖和大鼠子宫生长 ,而且这种作用可被tamoxifen抑制。丰水期水源水水质比枯水期水源水水质好 ;丰水期水质是长江优于嘉陵江 ,枯水期水质则是嘉陵江优于长江。丰水期水源水经水厂处理后 ,对MCF/ 7细胞增殖的刺激作用均增加 ,而枯水期则相反。枯水期A厂和B厂的出厂水水质是较好的。结论 某市饮用水中存在雌激素污染物 ,但尚不构成对人体的危害。
Objective To investigate the state of estrogenic pollutants in drinking water. Methods The organic pollutants were extracted from drinking water with solid-phase extraction (SPE). The estrogenic activity was evaluated with MCF/7 cell proliferation assay and uterotrophic assay, and estrogenic pollutants were detected by gas chromatography-mass spectrometric (GC/MS) analysis. Results The results by GC/MS analysis showed there were more than 100 kinds of pollutants in raw water, and more than 80 in treated water. The detected rates of bis (2-methylpropyl) phthalate and dibutyl phthalate which were the suspect estrogen were higher than 90%. In addition, the phenols (for example: 2,4-dimethyl phenol) were found in water samples. The organic pollution of raw water in Jialingjiang River was more severe than that in Yangtze River, and the organic pollution of treated water in summer was more severe than that in winter. The quality of treated water in C and E waterworks was better. The biological evaluation showed that the organic pollutants in raw water and treated water stimulated the proliferation of MCF/7 cells and the growth of uterine in rats, and tamoxifen suppressed the proliferation induced by the organic pollutants. The quality of raw water in summer was better than that in winter, and that in Yangtze River was better than that in Jialingjiang River in summer, and the contrary in winter. The effects of pollutants on MCF/7 cells proliferation in treated water were stronger than those in raw water in summer, but weaker in winter. The quality of treated water in A and B waterworks in winter was better. Conclusion The study implies that there are estrogenic pollutants in the drinking water in this city, but they are not harmful enough to the citizens.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第19期1751-1754,共4页
Journal of Third Military Medical University
基金
国家教育部 98"春晖计划"项目 ( 1999 36 )
重庆市科技攻关项目( 1999 5772 )
国家科技部攻关项目( 2 0 0 3BA86 9C和 2 0 0 3DA90 3B0 3 0 2 )~~
