摘要
目的:探讨幽门螺杆菌(H pylori)在体外对人胆囊上皮细胞(HGBEC)的损伤作用. 方法:采用液体增菌及人胆囊上皮细胞原代培养研究H pylori 对HGBEC的损伤作用. 结果:从H pylori的生长曲线可知观察其他因素对HGBEC 的影响在细胞生长最旺盛期即培养的2-6 d最为合适, H pylori增菌上清液及菌体破碎液对培养的HGBEC形态学及上清酶学指标ALP(33.84±6.00 vs 27.01±4.67,P<0.05), LDH(168.37±20.84 vs 55.51±17.17,P<0.01),GGT (42.01±6.18 vs 25.34±4.33 P<0.01)有明显影响. 结论:H pylori增菌上清液及菌体破碎液对培养的HGBEC 有明显损伤作用.
AIM: To study the mechanism of Helicobacter pylori (H pylori) damage to human gallbladder epithelial cells (HGBEC). METHODS: H pylori isolated from gallbladder were cultured in liquid medium, different concentration superna-tants and sonicated extracts of H pylori cell were added into HGBEC in primary culture. The morphous changes of the HGBEC were observed and the levels of ALP, LDH, and GGT were also examined. RESULTS: According to the culture curve of HGBEC, it was convenient to study the changes of HGBEC by adding H pylori sonicated extracts and H pylori culture supenatents. Both of H pylori sonicated extracts and H pylori culture supenatents had significant influence on H pylori morphous, HGBEC grew slowly, viability decreased, and detachment increased. Furthermore, cell rupture and died. The levels of ALP ( 33.84 ± 6.00 vs 27.01 ± 4.67,P<0.05), LDH (168.37 ± 20.84 vs 55.51 ± 17.17,P<0.01) and GGT(42.01 ± 6.18 vs 25.34 ± 4.33,P<0.01) increased significantly in the HGBEC culture supernates, which was time-and concentration-dependent. The damage effects on HGBEC in H pylori cultured liquid were stronger than in H pylori sonicated extracts. CONCLUSION: The culture supernates and sonicated extracts of H pylori has obviously induced the damage to HGBEC.
出处
《世界华人消化杂志》
CAS
2004年第8期1835-1839,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.39970039~~
