摘要
本研究的目的是筛选和评价可用于薄片法血小板聚集试验的血小板激活剂。应用下列方法进行实验 :①测定常用血小板激活剂 ,包括ADP、胶原、肾上腺素、花生四烯酸和瑞斯托霉素及阳离子没食子酸丙酯溶液 (c PG)在塑料薄片上诱导 15名健康献血者血小板聚集强度和时间 ;②测定添加血小板抑制剂PGI2 、cAMP或EDTA前后常用血小板激活剂及c PG在诱导 15名健康献血者血小板聚集时间 ;③测定不同浓度肝素对c PG诱导的 15名健康献血者小板聚集时间的影响 ;④检测c PG诱导健康献血者不同浓度血小板的聚集强度和时间 ;⑤检测c PG诱导服用阿司匹林患者血小板聚集时间。结果表明 :①在塑料薄片上c PG诱导血小板的聚集强度最强 ,肉眼清晰可见 ,需要时间较短 ;②瑞斯托霉素、花生四烯酸和c PG均可检测出抑制剂PGI2 和cAMP对血小板的抑制 ,其中c PG检测能力相对最强 ;③ 0 .5 - 3U ml肝素对c PG诱导的血小板聚集时间无明显影响 ;④健康献血者血小板稀释至 30× 10 9 L仍可被c PG诱导出明显的肉眼可见的聚集 ;⑤c PG诱导服用阿司匹林患者血小板聚集时间明显延长。结论 :与常用血小板激活剂比较 ,c PG应用于薄片法血小板聚集试验中具有明显优势。
The study was purposed to explore the suitalbe platelet ac tivators to be used in slide platelet aggregation test. Experiments were as follows: (1) to detect the intensity and time in 15 healthy donors′ platelet aggregation tests induced by cationic propyl gallate (c-PG) and the usual plat elet activators: ADP, collagen, epinephrine, arachidonic acid and ristocentin, r especti vely; (2) to detect the time in platelet aggregation tests of 15 healthy donors i nduced by c-PG and the above usual platelet activators respectively after a ddition of PGI 2, cAMP or EDTA; (3) to detect the time in 15 healthy donors′ p latelet aggregation tests induced by c-PG after addition of heparin; (4) to det ect the intensity and time of platelet aggregation induced by c-PG at the platelet co unt of (240-15)×109/L, (5) to detect the time of platelet aggregation induced by c -PG in eight patients each of whom had taken 100 mg aspirin per day for five da ys. The results showed that (1) c-PG reduced the strongest intensity of plate let aggregation and the time taken was appropriate, (2) c-PG was the most effective activator to reveal the inhibitive effect on platelet by PGI 2, cAMP or EDTA, ( 3) 0. 5-3 U/ml heparin did not significantly change the platelet aggregation induced b y c-PG, (4) 15 healthy donors′ platelet aggregation induced by c-PG displayed clearly o n the slide until the platelet count below 30×109/L, (5) The platelet aggrega tion time induced by c-PG was significantly prolonged in eight patients who had taken aspirin. In conclusion, compared to the usual platelet activators, c-PG has remarkable potential advantages when used in slide platelet aggregati on test.
出处
《中国实验血液学杂志》
CAS
CSCD
2004年第5期680-683,共4页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目 (编号 3 0 2 714 43 )
关键词
血小板
聚集功能
激活剂
platelet
aggregation
platelet activator
