摘要
采用室内培养测定方法,研究了对苯二甲酸降解菌CandidaCP02胞内海藻糖的抗渗透压冲击作用,同时分析了CP02在降解不同浓度对苯二甲酸时,胞内海藻糖含量的变化以及海藻糖酶和海藻糖-6-磷酸合成酶活性的差异。结果表明,随着对苯二甲酸浓度的增加,海藻糖合成酶的酶活也相应升高,胞内海藻糖含量迅速提高,有利于细胞的存活和对苯二甲酸的降解。
We have found that high concentration of intracellular trehalose w a s able to resist high load, which prevent microbes from harm and indicated that Candida strain CP02 isolated in our laboratory had a great degradative capabilit y of terephthalic acid (TA). The effects of intracellular trehalose concentratio n in CP02 on high osmosis tolerance were studied and exhibited that: with additi on of 5%NaCl into medium, trehalose content was nearly 3 times more than that in culture medium without containing NaCl. It demonstrated the concentration of trehalose increased along with osmosis tolerance. We also measured the contents of trehalose accumulated, trehalose and trehalose-6p synthase activity during the degradation of different original TA. In the whole process (0～120 h) treh alose content was capable of achieving two fastigiums, which were induced by TA concentration and scarcity of nutrition, respectively. Trehalose content was up to 5%in 1 000 mg·L-1 TA solution after cultivating 36 h, however, trehalo se content in 0 mg·L-1 TA solution was only 2%at the same time. Trehalose-6-p synthase activity in 1 000 mg·L-1 TA solution was six times more th an that in blank TA solution. These date showed that trehalose-6-p synthase activity and the intracellular trehalose concentration increased in response to the increase of TA concentration,which may protect cell viability of TA. The c orrelation study between intracellular trehalose metabolism of CP02 and TA degra dation was found to be in favor of TA removal rate.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2004年第5期977-980,共4页
Journal of Agro-Environment Science
基金
南京市科技发展项目(20022035)
关键词
海藻糖
海藻糖合成酶
对苯二甲酸
降解
trehalose
trehalose-6p synthase
terephthalic acid
degradation
