摘要
目的检验大剂量葡萄糖水平对培养的原代人视网膜毛细血管内皮细胞(humanretinalcapillaryendothelialcells,HRCEC)的直接影响。方法HRCEC细胞从捐赠人眼中分离得到。细胞在含有5mmol/L或25mmol/L葡萄糖的培养基中培养6d。应用锥虫蓝染色测定细胞活力、流式细胞计数分析细胞周期、TUNEL分析细胞凋亡。结果HRCEC细胞在25mmol/L葡萄糖中培养6d,细胞活力显著下降。通过流式细胞计数和TUNEL测定,凋亡细胞的数目在大剂量葡萄糖培养基中显著升高。结论大剂量葡萄糖可诱导人视网膜毛细血管内皮细胞凋亡,这有可能是促进糖尿病性视网膜病发展的原因。
Objective To examine the direct effect of high glucose le ve ls on primary cultured human retinal capillary endothelial cells (HRCEC). Methods HRCECs were isolated from donated eyes and cultured for 6 days in the m edia containing 5 or 25 mmol/L glucose. The cell viability was determined by try pan blue exclusion assay and cell cycle analyzed by flow cytometry, with the ce ll apoptosis assayed by TUNEL method. Results The cell viability was significant ly decreased after exposure to 25 mmol/L glucose, and the number of apo ptotic cells determined by flow cytometry and TUNEL was significantly increased in response to high-dose glucose treatment. Conclusion High-dos e glucose induces apoptosis in HRCEC, which may contribute to the developme nt of diabetic retinopathy.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第5期435-438,共4页
Journal of First Military Medical University
基金
教育部回国人员启动基金(2003)
中山大学回国人员启动基金~~
