摘要
以中国普通野生稻初级核心种质中广西普通野生稻部分中的 2 2 3份野生稻为材料 ,以平均分布于水稻 12条染色体上的 34对SSR引物和中国稻种资源目录中的表型性状分析广西普通野生稻SSR位点的等位变异、多样性的地理分布及不同生长习性间的多样性分布等。结果表明 ,每对引物检测到的多态性片段 7～ 4 8条 ,平均为 2 4 .91条 ,普通野生稻的等位变异数明显大于地方稻种 ,在所分析的SSR位点中杂合位点比例变化在 1.35 %～ 81.31%之间 ,平均为 32 .0 1% ,与自花授粉的栽培稻相比具有较高的杂合率 ;北纬 2 2°～ 2 3°和 2 3°～ 2 4°范围内的两个区域内(一个包括隆安、扶绥和邕宁三县 ,另一个包括象州、来宾、武宣、玉林和贵港五个县 )所包含的普通野生稻数量多 ,遗传多样性大 ,在DNA水平上是广西普通野生稻的遗传多样性中心 ,而表型性状多样性中心是在北纬 2 1°～ 2 2°和2 2°～ 2 3°,其多样性分布与DNA水平不完全一致。在 4种生长习性间 ,DNA水平上的遗传多样性大小依次为匍匐型 ,倾斜型 ,半直立型和直立型 ,表型水平的多样性与DNA水平的多样性基本一致。
In this paper,allelic variation and genetic diversity among geographies and growth habit types were studied by using 223 accessions of common wild rice primary core collection in Guangxi Province,with 34 SSR primers locating on 12 chromosomes of rice and 19 phenotypic traits.In the results,24.91 alleles were detected per locus on average with a range from 7 to 48.Compared to the cultivated rice,the wild rice showed more allelic variations.The ratio of heterozygote of SSR locus was 32.01% on average,and it’s range was 1.35% ～ 81.31%.The frequency of heterozygote of SSR locus in Oryza rufipogon Griff was much higher than in Oryza sativa L.The geographical distribution of genetic diversity measured by SSR markers was not completely accordant with that by phenotypic traits.At DNA level,more wild rice individuals and higher genetic diversity were included within the area covering north latitude 22°～23° and 23°～24°(comprising Longan,Fusui,Yongning,Xiangzhou,Laibin,Xuanwu,Yulin and Guigang county),which formed the center of genetic diversity.But the center of genetic diversity at the phenotypic level located within north latitude 21°～22° and 22°～23°.Among the four growth habit types,the genetic diversity from high to low was found respectively in prostrate type,sloping type,slighting type,and erect type at both DNA and phenotypic levels.
基金
国家重点基础研究发展规划 ( 973计划 )项目资助 (编号 :G19980 10 2 0 1)~~
关键词
普通野生稻
表型性状
SSR标记
遗传多样性
common wild rice ( Oryza rufipogon Griff)
phenotypic trait
SSR marker
genetic diversity
