摘要
目的:探讨实验室条件下人体角质形成细胞的培养技术,为角质形成细胞的多方面研究提供可靠的细胞来源。方法:采用改良的无血清培养技术进行人体角质形成细胞的培养,用显微镜及电子显微镜观察角质形成细胞的形态特征。结果:培养的角质形成细胞在多次传代后仍保持了正常的形态。结论:用改良的培养角质形成细胞的技术可为角质形成细胞进一步的实验和临床提供可靠的、丰富的细胞来源。
Objective To explore the cultivating technique of human keratinocyte under laboratory conditions, and to provide reliable cells resource for the experimental study on keratinocyte in many ways. Methods The improved cultivating technique of human keratinocyte with non-serum was used and microscope and electron microscope were used to observe keratinocyte morphological characteristics. Results The cultivated keratinocyte was still to remain normal morphological characteristics after many times transfer of culture. Conclusion The improved cultivating technique of human keratinocyte can provide reliable and abundant cells resource for the farther study on keratinocyte in experiment and clinic.
出处
《中国美容医学》
CAS
2004年第5期522-524,i001,共4页
Chinese Journal of Aesthetic Medicine
基金
国家高技术研究发展计划(863)资助项目(2001AA215361)
