摘要
目的:建立用气相色谱的顶空进样法测定盐酸头孢吡肟中N-甲基吡咯烷残留量。方法:用HP-5,0.53 mm×30 m色谱柱,以氮气为载气,FID检测器,顶空进样,通过外标法计算盐酸头孢吡肟中N-甲基吡咯烷的残留量。结果:盐酸头孢吡肟中检出N-甲基吡咯烷,标准曲线的线性范围为43.4-347.2μg·mL-1,相关系数为0.9997;定量限为4.17μg·mL-1;平均加样回收率为98.8%,RSD为2.8%。结论:本法快速、简便,灵敏度高,分离度好。
Objective: To establish a method for determining residual N - methylpyrrolidine of cefepime dihydro chloride by headspace GC. Methods:The GC was equipped with a flame - ionization detector for headspace injecting. A 0. 53 mm ×30 m HP - 5 chromatographic column was adopted. The carrier gas was nitrogen. Assay of residual N - methylpyrrolidine was calculated according to the external standard method. Results:N - methylpyrrolidine was found in the sample of cefepime dihydrochloride. The standard curve was linear over the range of 43. 4 -347. 2 μg·mL-1 (r =0. 9997). The limit of the quantifying was 4. 17 μg·mL-1. The average recovery was 98. 8% (RSD = 2. 8%,n=6). Conclusion: The method is rapid, accurate and suitable for the determining of residual N - methylpyrrolidine in cefepime dihydrochloride.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2004年第4期432-433,共2页
Chinese Journal of Pharmaceutical Analysis
