摘要
脉冲电泳是一种可用于分离 2 0Kb～ 1 0Mb大分子量DNA的新型凝胶电泳技术 ,已广泛用于多种酵母和丝状真菌的核型分析及核型多态性分析的研究。本文在介绍脉冲电泳工作原理的基础上 ,对它在真菌分类鉴定及遗传和分子研究方面的应用作了简要的综述。
Pulsed Field Gel Electrophoresis (PFGE) is a new type technique of gel electrophoresis which can be used to (separate) large DNA molecules ranging from 20Kb to 10Mb. It has been widely applied to the karyotype analysis and karyotype (polymorphism) for various yeasts and mycelial fungi. In this paper, the principle of PFGE and its application in the research fields of (classification), genetics and molecule of fungi have been discussed.
出处
《贵州农业科学》
CAS
2004年第5期82-84,共3页
Guizhou Agricultural Sciences
