摘要
目的 :对知母宁体外抗单纯疱疹病毒Ⅰ型的活性进行研究。方法 :采用MTT比色法测定知母宁对Vero细胞的毒性作用。采用中性红染色法 ,以阿昔洛韦为阳性对照药物 ,考察不同给药方式与不同药物处理时间下知母宁体外抗单纯疱疹病毒Ⅰ型 (HSV ⅠSm4 4 )的活性。结果 :MTT法测得Vero细胞对知母宁的最大耐受浓度与半数中毒浓度 (TC50 )分别为0 .2 9与 3.6 6mg·ml-1。不同加药方式下 ,知母宁与阿昔洛韦均对HSV ⅠSm4 4表现出综合抑制作用并能抑制病毒吸附后的复制增殖过程 ,这些作用总体上随药物浓度升高而增强 ,其中知母宁抗病毒有效率 (ER % )最高达 76 .4 2 %。知母宁预防HSV Ⅰ病毒吸附的作用较弱 ,但其在高浓度 (2 .0 8mg·ml-1以上 )对HSV Ⅰ具强的直接杀伤作用 ,最高ER %值为 98.86 %。随药物处理时间延长 ,低浓度知母宁抗HSV Ⅰ的ER %值递减 ,2 .0 8mg·ml-1以上知母宁的ER %值变化小。在不同药物处理时间下 ,知母宁及阿昔洛韦组病毒的感染性均较平行设置的病毒对照组减弱 ,且知母宁组的感染力较阳性药物组更弱。知母宁组病毒感染力下降值△lgTCID50 随处理时间增加而增加。结论
Objective: To study the activity of chinonin against HSV Ⅰ in vitro . Method: The toxicity of chinonin on Vero cell was measured by MTT colorimetry. The activity of chinonin of different drug addition methods and drug treating time against HSV Ⅰ Sm44 was studied in vitro by using neutral red staining method, in acyclovir served as positive reference drug.Result: By assay of MTT method, Vero cells' maximal tolerant concentration and the median toxic concentration (TC 50 ) to chinonin was 0.29 and 3.66 mg/ml, respectively. Both chinonin and acyclovir were found to have comprehensive inhibitive effect against HSV Ⅰ Sm 44 by different drug addition methods, and they inhibited the proliferation process of virus after adsorption. Generally, these effects became stronger with the increase of concentration, and the highest anti viral effective rate (ER%) of chinonin was 76.42% . The preventive effect of chinonin against HSV Ⅰ adsorption was weak, but it was observed to have a strong and direct inhibiting effect to the virus in the concentration higher than 2.08 mg/ml with the highest ER% of 98.86% . With the time prolongation of pharmacotherapy, the anti viral ER% value of chinonin decreased in concentration decreased progressively. However, it showed a small change in the concentration ≥ 2.08 mg·ml -1 . After different pharmacotherapy time, the virus infectivity of both chinonin and acyclovir group was weaker compared with that of the parallel virus controlled group, while the infectivity of chinonin group was lower than that of acyclovir group. The decline of virus infectivity of chinonin group (△lg TCID 50 ) increased with the prolongation of pharmacotherapy time.Conclusion: Chinonin has a activity for strong inhibition of HSV Ⅰ at several effect points in vitro .
出处
《中国药师》
CAS
2004年第9期666-670,共5页
China Pharmacist
