摘要
【目的】探讨血管紧张素-(1-7)犤Ang-(1-7)犦对异丙肾上腺素所致大鼠急性心肌缺血的保护作用。【方法】96只Wister大鼠随机分为对照组、培哚普利组、Ang-(1-7)组、培哚普利+Ang-(1-7)组,每组24只。异丙基肾上腺素10mg·kg-1腹腔内注射以制备急性心肌缺血动物模型,同时,治疗组分别以培哚普利2mg·kg-1·d-1口服灌注及Ang-(1-7)经植入式微泵以25μg·kg-1·h-1持续静脉注入;对照组以等量生理盐水输注。分别在第48h、第7天和第14天各处死8只,处死前检测颈动脉血压、心率;采血测定肌酸激酶、乳酸脱氢酶、超氧化物歧化酶;计算心肌梗死总体积占全心肌体积的百分比。【结果】异丙基肾上腺素导致心肌梗死、血压升高、心率增快,肌酸激酶、乳酸脱氢酶增高,超氧化物歧化酶下降,P<0.05;48h后,培哚普利和Ang-(1-7)治疗组心肌梗死范围缩小、血压回落、心率减慢,肌酸激酶、乳酸脱氢酶下降,超氧化物歧化酶上升,这种优势随治疗时间延长而明显。治疗第14天,对照组、培哚普利组、Ang-(1-7)组、培哚普利+Ang-(1-7)组心肌梗死总体积占全心肌体积的百分比分别为(9.8±0.6)%,(5.9±0.5)%,(5.6±0.3)%,(4.2±0.3)%,与对照组相比,差异有统计学意义,P<0.05;培哚普利组和Ang-(1-7)组间比较显示Ang-(1-7)与培哚普利无差异。
To investigate the protective effects of angiotensin (1 7) [Ang (1 7)] in acute myocardial ischemia induced by isoprenaline in rats. Ninety six Wister rats were randomized to four groups as follow: ①control, ②perindopril, ③Ang (1 7), ④perindopril+Ang (1 7), 24 rats in each group. Peritoneal injection with isoprenaline (10 mg·kg-1) to prepare the model of acute myocardial ischemia, at the same time, perindopril was started orally with 2 mg·kg-1·d-1 and intravenous infusion of angiotensin (1 7) was started continuously with 25 μg·kg-1·h-1 by implanted osmotic minipumps in the treatment groups, isometric saline was started intravenously in control group. Forty eight hours, 7 days, and 14 days after treatment, 8 rats were killed separately, the carotid artery systolic and diastolic blood pressure, heart rate were measured before executing, and plasma was taken for determination of creatine kinase (CK), lactic dehydrogenase (LDH), superoxide dismutase (SOD), at last the percentage of total myocardial infarction volume in whole myocardium volume was measured. Isoprenaline induced area of myocardial infarction,blood pressure,heart rate, CK,LDH increased, and SOD decreased, P< 0.05. After 48 hours treatment, area of myocardial infarction, blood pressure, heart rate, CK and LDH decreased, and SOD increased in perindopril and Ang (1 7) groups, this advantage was obviously significant with the treatment time prolonged. In 14 days, the percentage of total myocardial infarction volume in whole myocardium volume in control, perindopril, Ang (1 7), and perindopril+Ang (1 7) groups were (9.8±0.6)%,(5.9±0.5)%,(5.6±0.3)%, and (4.2±0.3)%, respectively. Compared to the control group, the difference was significant, P< 0.05. There was no difference between perindopril and Ang (1 7) groups using single drug, but perindopril+Ang (1 7) group (both drugs using together) had synergistic effect. [Conclusion]Angiotensin (1 7) has the effect of myocardial protection. Its efficacy is the same of perindopril. The effect is more prominent in using both drugs.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2004年第4期326-329,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
高等学校博士学科点专项科研基金资助项目(20020558064)
