摘要
目的 探讨中药复方益气解毒颗粒对鼻咽癌细胞杀伤作用的分子机制。方法 筛选益气解毒颗粒药液对鼻咽癌杀伤的半抑制浓度(IC_(50)),用0.1倍半抑制浓度(0.1×IC_(50))的益气解毒颗粒作用鼻咽癌细胞HNE1 96h,用固相pH梯度双向凝胶电泳分离益气解毒颗粒处理和未处理的鼻咽癌细胞HNE1的总蛋白质,银染显色,PDQuest 2-de软件分析差异蛋白质点。结果 益气解毒颗粒药液杀伤鼻咽癌细胞HNE1的半抑制浓度为6.25mg/ml;双向电泳图像分析益气解毒颗粒药物处理组图像的平均蛋白质数为1120±89,未处理组的平均蛋白质数为1281±102;差异蛋白质有673个,其中有218个表达增强,有455个蛋白质表达下降。结论 益气解毒颗粒对鼻咽癌细胞的杀伤作用这些差异表达蛋白质有关,这些差异蛋白质有待质谱分析鉴定,为中药作用的分子机理研究提供了新的思路。
Objective To investigate the molecular mochanism of Qi-Boosting
Toxin Resolving Granule (QBTRG)in the inhibition of nasopharyngeal carcinoma cell
line HNE1 based on the techniques of proteomics. Methods The IC_(50) of QBTRG
killing HNE1 cells was determined by MTT at first. Then, HNE1 cells were treated with
QBTRG at the concentration of 0.1×IC_(50) for 96 hours. After this step, the cells were
collected and the total protein was extracted from these cells. By two-dimension clec-
trophoresis, the proteins were separated and protein profiles were obtained. Followed
was an analysis on the differently expressed proteins by PDQuest2-de microsolft on the
basis of protein profile comparison between two groups of HNE1 cells treated with QB-
TRG and those not treated at all. Results The IC_(50) of QBTRG on NHE1 cells was
6.25mg/ml. The mean number of protein spots was 1120±89 for treated HNE1 cells
with QBTRG and that was 1281±102 for the untreated HNE1 cells. Therefore, the dif-
ferently expressed protein spots were 673, among them 218 being up-regulated and 455
being down-regulated in their expression in treated HNE1 cells. Conclusions The in-
hibitory effects of QBTRG on nasopharyngeal careinoma cells may be closely associated
with the pattern of these differently expressed proteins. The physical and chemical prop-
erties of these proteins can be identified by the means of MS. Then, the data got from
this identification should be helpful for the further investigation on the molecular mecha-
nism of QBTRG in the inhibition of this kind of cancer cells.
出处
《中国中西医结合耳鼻咽喉科杂志》
2004年第3期120-123,共4页
Chinese Journal of Otorhinolaryngology in Integrative Medicine
基金
国家自然科学基金(39770933
30171174
30271678)
国家中医药管理局资助项目(95B063
02-03JP29)
湖南省教育厅重点资助项目(01A014)
关键词
益气解毒颗粒
鼻咽癌
细胞HNE1蛋白质
表达
双向电泳
Nasopharyngeal carcinoma
Proteomics
Two-dimensional electrophoresis
Qi-Boosting Toxin-Resolving Granule
