摘要
将油菜素内酯(brassinosteroids,BR)超敏感受体激酶基因Sud1与水稻抗病受体激酶基因Xa21经体外点突变后构建了带SUD1LRR-JM结构域与XA21激酶域(kinase)的合成受体(chimera)基因SNRG1,同时构建了SUD1LRR突变体对照受体基因SNRGmL和XA21kinase突变体对照受体基因SNRGmK.应用基因枪(particlebombardment)转化技术成功将SNRG1,SNRGmL和SNRGmK导入粳稻模式品种台北309的胚性愈伤组织中,并由此获得了再生植株和细胞株.经PCR、Northernblot分子检测和表型观察证实外源基因已经转入并整合到水稻基因组.防卫基因表达的结果表明SNRG1可能已在体内微量的BR作用下,激发了水稻的防卫反应.该结果为进一步研究XA21激酶的抗性激发奠定了基础.
A chimeric receptor kinase gene SNRG1 was constructed by combining the Arabidopsis SUD1 (brassinosteroid (BR) supersensitive receptor kinase) LRR-JM domain with the rice XA21 (disease resistance receptor kinase) kinase domain with point mutagenisis in vitro. The SUD1 LRR mutant SNRGmL and the XA21 kinase mutant SNRGmK were also constructed as controls respectively. Using particle bombardment, SNRG1, SNRGmL and SNRGmK were successfully transferred into rice embryogenic calli of japonica variety Taipei 309. Transgenic rice plants and cell lines were confirmed by PCR, Northern blot and transformant phenotype. Preliminary result showed that rice defense pathways could have been activated by trace amount of endogenous BR in the SNRG1 transgenic plants. These results provided the foundation for further investigation of XA21 kinase activation upon BR ligand binding.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2004年第5期482-486,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家杰出青年基金资助项目(30125030)
国家"863"资助项目(2001AA222321)
中国科学院创新资助项目(KSCX2-SW-301-02).
