摘要
根据已发表的IBDV各致病型毒株的序列,在VP5和VP2的重叠基因区设计合成了一对寡核苷酸引物,对2株参考毒株和8份疑似IBD的临床病料进行RT-PCR检测均得到了明显的阳性扩增结果;而对作为阴性对照的常见4种鸡病病原:鸡新城疫病毒、鸡传染性支气管炎病毒、鸡马立克氏病病毒、大肠杆菌均没有扩增到任何片段。利用琼脂扩散试验进行IBDV病原常规鉴定,结果证实5份病料呈现IBD阳性。表明建立的IBD的RT-PCR诊断技术具有快速、特异、敏感的特点。
The primers Ined in the overlapping area of VP5 and VP2 were designed and a reverse transcription polymerase chain reaction (RT-PCR) was developed. Two vaccine strains and eight field bursasl samples were amplified successfully by the primers. Four pathogens which can result in other chicken disease showed the negative results. However, through agar gel precipitation, the result was that 5 samples were positive. It indicated that RT-PCR is a rapid and sensitive diagnosis method for IBD.
出处
《动物医学进展》
CSCD
2004年第5期82-84,共3页
Progress In Veterinary Medicine
基金
安徽省教育厅青年基金项目(2003jq111)
