摘要
从化工厂污泥中分离到一株酵母菌 ,初步鉴定为德氏酵母属 (Debaryomycessp ) ,命名为Gb。该菌株可以在 0～ 2 0 %Na2 SO4环境中生长 ,并能以苯酚为唯一碳源和能源。其苯酚降解最适浓度为 1 0 0 0mgL-1 ,最适Na2 SO4浓度为 5 %,最适pH和温度分别为 5 5和 30℃ ,在 2 5 0ml三角瓶中最适装液量为 1 0 0ml。当Na2 SO4盐浓度为 5 %、苯酚浓度为 1 0 0 0mgL-1 时 ,最适条件下培养 3天即能降解 95 %的苯酚。本文还对其耐盐机理进行了初步研究 ,发现细胞内海藻糖含量随外界盐浓度增加而增加 ,表明Debaryomycessp 在高盐条件下能以海藻糖作为渗透调节物质。
A yeast strain Gb identified as Debaryomyces sp. was isolated from sludge of a chemical plant by acclimatization . This strain was capable of growing in media with 20% Na_2SO_4 and utilizing phenol as sole carbon and energy sources. Effects of environmental factors on growth of this strain and degradation of phenol were tested. It was found that the optimal condition for growth and phenol degradation of this strain was phenol 1 000 mg L -1, Na_2SO_4 5%, pH 5.5 and temperature 30℃. The optimal volume is 100 ml in a 250 ml conic flask. This strain can degrade 95% phenol in 3 days when Na_2SO_4 was 5% and phenol was 1 000 mg L -1. The osmoregulation mechanism of this strain was studied. It was found that the intracellular level of trehalose increased with the increase of salinity, indicating that Debaryomyces sp. can use trehalose as osmoprotectant in hypersaline environment. The physiological characteristics of the strain were also studied, yielding some basic parameters useful for biodegrading of high salt concentration phenol wastewater.
出处
《土壤学报》
CAS
CSCD
北大核心
2004年第5期756-760,共5页
Acta Pedologica Sinica
基金
高校博士点专项资助基金 (2 0 0 0 0 3 0 711)
江苏省环境保护厅资助项目 (苏环科 2 0 0 0 (2 1)号 )
