摘要
利用含HAL1基因的高效植物表达载体pAHF转化根癌农杆菌 (Agrobacteriumtumefaciens,LBA4 4 0 4 ) ,用农杆菌介导的叶圆盘法对烟草 (NicotianatabacumL .)叶片进行遗传转化。在含卡那霉素的MS分化培养基上选择转化体和植株再生 ,转化植株可在含卡那霉素的生根培养基中生根。经PCR检测发现 ,在 8个转化子中有 6个转化子获得特异性扩增条带。耐盐试验表明 ,在被检测的 90 0株转化子中有 4 81株可在含 0 .80 %～ 1.5 0 %NaCl的生根培养基中生根 ,生根率为 5 3.4 5 % ,对照烟草植株只在低于 0 .80 %NaCl的生根培养基中生根 ,生根率为 73.75 %。由此证明HAL1基因的转入提高了烟草的耐盐性。
The HAL1 gene cloned from yeast was inserted into a binary vector (pAHF) and was further transformed into Agrobacteriun tumefaciens (LBA4404). Then LBA4404 was again transformed into tobacco (Nicotiana tobacum) plants by leaf disc transformation. The transformants were selected on the MS culture medium containing Kanamycin. PCR analysis showed that 6 of 8 transformants obtained special amplification band. The result of salt tolerance tests indicated that 481 of 900 transformants took root on 0.80%～1.50% NaCl culture medium with rooting rate of 53.45%, while the control only took root on NaCl culture medium under the content of 0.80% with rooting rate of 73.75%. It is concluded that the HAL1 gene has integrated into tobacco genomes and its expression in tobacco could enhance the salt tolerance of transgenetic tobacco.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2004年第4期47-49,共3页
Journal of Northeast Forestry University
基金
国家 973项目 (G1 99990 1 60 0 3)资助
