摘要
目的研究咳嗽变异性哮喘气道重塑及可能机制。方法选择典型哮喘15例,咳嗽变异性哮喘12例,正常对照组8例行纤维支气管镜检查,获取段支气管黏膜组织作病理切片,光镜下测定各组基底厚度,同时作支气管肺泡灌洗,测定肺泡灌洗液(BALF)中基质金属蛋白酶(MMP-9)含量。结果典型哮喘组基底膜厚度为(9.05±1.67)μm,咳嗽变异性哮喘组为(7.27±1.6)μm,均明显高于正常对照组(5.06±0.67)μm(P<0.05),两哮喘组间也有明显差异。典型哮喘组MMP-9含量为(5.08±1.46) mg/g 蛋白,咳嗽变异性哮喘组为(4.5±1.64)mg/g蛋白,明显高于正常对照组(2.01±0.9)mg/g蛋白(P<0.05),但两哮喘组间差异无显著性。各组基底膜厚度与MMP-9含量之间相关分析提示有明显相关性(r=0.632)。结论咳嗽变异性哮喘存在着气道重塑,基质金属蛋白酶MMP-9可能参与了气道重塑过程。
Objective:To study the mechanism of airway remodeling in cough-variant asthma.Methods:Bronchal biopsy specimens were obtained from15asthmatic patients,12cough-variant asthmatic patients and eight healthy subjects,the basement membrance thickness was measured with light microscopy.At the same time,Samples of bronchoalveolar lavage fluid from27asthmatic patients(include12cough-variant patients)and from control subjects ware assessed for MMP-9.Results:The basement membrane thickness was significantly higher in participants with cough-variant asthma[mean7.27μm(SD1.6),P=0.008]and classic asthma[9.05μm(1.67)P<0.001)]than in controls[5.06μm(0.67)]and was significantly greater in participants with classic asthma than in those with cough-variant asthma(P=0.012);the MMP-9levels were significantly increased in cough-variant asthma and classic asthma than in control subjects(P<0.05),the thicking of basement membrane was significantly associated with the MMP-9levels.Conclusions:The basement membrane thickening occurs in cough-variant asthma,MMP-9may play a role in airway remodeling in asthma.
出处
《中国内镜杂志》
CSCD
2004年第8期13-14,17,共3页
China Journal of Endoscopy
关键词
咳嗽变异性哮喘
气道重塑
cough-variant asthma
airway remodeling
