摘要
目的 :优选测定银杏黄酮含量时的水解时间。方法 :用甲醇 - 2 5 %盐酸 (4∶1)的混合液 2 5ml热回流水解后稀释 ,用HPLC法测定银杏黄酮含量。以槲皮素、山萘素和异鼠李素为对照品 ,甲醇 - 0 .4 %磷酸 5 0∶5 0为流动相 ,ShimpackCLC ODS(15 0mm× 6mm)为固定相 ,检测波长为 36 0nm ,柱温 30℃。结果 :银杏叶、银杏叶粗提物、银杏叶精制物水解 2 .0h时银杏黄酮的检测含量最高。结论 :用甲醇 - 2 5 %盐酸 (4∶1)的混合液热回流测定银杏叶和银杏叶提取物的黄酮含量时水解 2 .
AIM: To select proper hydrolysis time to determine Ginkgo biloba flavonoids content. METHODS: Sample dissolved in 25mL MeOH-25%∶HCl(4∶1) mixture and was heated to hydrolyze for various time, then diluted. Flavonoids'content was determined by HPLC. The conditions were developed by Shimpack CLC-ODS(150mm×6mm) column using 0.4% phosphoric acid solution-Methanol(50∶50), the detective wavelength was at 360nm with quercetin、kaempferol and isorhamnetin as standards, temperature being 30℃. RESULTS: Contents of flavonoids of Ginkgo biloba leaves,Ginkgo biloba crude extracts and Ginkgo biloba refined extracts were highest in the hydrolysis time of 2.0h. Ginkgo biloba flavonoids can be completely hydrolyzed in 2.0h. CONCLUSION: The hydrolysis time of 2.0h is best.
出处
《中成药》
CAS
CSCD
北大核心
2004年第8期662-664,共3页
Chinese Traditional Patent Medicine
作者简介
邓丹丹(1978～),女,青海西宁市人,硕士,研究方向:中药制剂,电话:025-5322007.
