摘要
目的 :观察L 精氨酸和一氧化氮对内毒素性肺损伤的影响。方法 :采用静脉注射脂多糖 (LPS)制备内毒素性肺损伤大鼠模型。将 32只SD大鼠随机分为 4组 :空白对照组、LPS模型组、L 精氨酸高剂量 (5 0 0mg/kg)和低剂量 (2 5 0mg/kg)治疗组 ;实验过程中监测大鼠平均动脉压 (MAP) ,定时测定血浆中NO含量 ,观察LPS引起大鼠急性肺损伤后肺系数、肺水肿情况和肺组织中丙二醛 (MDA)含量、一氧化氮合酶 (NOS)、超氧化物歧化酶 (SOD)活性的变化 ,以及L 精氨酸对内毒素性肺损伤的治疗作用。结果 :L 精氨酸可明显降低肺系数和肺含水量 ,减少MDA含量 ,增强SOD活性 ,明显改善LPS引起的肺组织细胞损伤。结论 :L 精氨酸对内毒素性肺损伤具有治疗作用。
AIM:To investigate the effects of L-arginine and nitric oxide(NO) on endotoxin induced lung injury in rats. METHOD: Thirty two healthy male SD rats weighing 300±20 g were used. Common carotid artery (CAA) and common carotid vein (CAV) were exposed. Mean arterial pressure (MAP) was measured through a pressure transducer connected with intubation of CAA. The animals were randomly divided into four groups:group 1:control;group 2:LPS (5 mg/kg intravenous injection,iv);group 3:high dose L-arginine (500 mg/kg intraperitoneal injection,ip) and group 4:low dose L-arginine (250 mg/kg ip). Group 1:0.9% saline solution was given and the animals were killed 6 h after given the saline solution. Group 2:0.9% saline solution was given 3 h after LPS iv and the animals were killed 3 h after medication. Group 3 and 4:L-arginine was given 3 h after LPS iv and the animals were killed 3 h after medication respectively. The pulmonary coefficient and water content in pulmonary tissue were calculated (%).The NO content in plasma,MDA content and NOS,SOD activity in the pulmonary tissue were measured. RESULT:L-arginine significantly decreased pulmonary coefficient and water content in pulmonary tissue and ameliorated endotoxin induced lung injury. L-arginine significantly decreased MDA content and inhibited NOS activity and enhanced SOD activity in the pulmonary tissue. CONCLUSION:It may be concluded that L-arginine has beneficial effect on lung injury induced by LPS.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2004年第4期349-352,共4页
Journal of China Pharmaceutical University
基金
国家人事部留学人员重点资助项目 (No .990 0 789)
河北省博士基金资助项目 (No .995 470 15D)~~
关键词
肺损伤
一氧化氮
L-精氨酸
脂多糖
内毒素
Lung injury
Nitric oxide
L-arginine
Lipopolysaccharide
Endotoxin
