摘要
目的 :观察磷酸化的分裂原激活的蛋白激酶 (p38MAPK)在脂多糖 (LPS)引起的大鼠急性肺损伤中在体原位的表达 .方法 :采用免疫组织化学ABC法和Western blot技术 ,检测磷酸化的 p38MAPK在大鼠急性肺损伤模型气道和肺组织的表达 ,并同时观察N 乙酰半胱氨酸 (NAC)对其表达的影响 .结果 :对照组气道和肺组织无或偶见反应极弱的p38MAPK阳性细胞 ,散在分布于气道上皮细胞和肺泡上皮细胞 .LPS致伤组 p38MAPK阳性细胞较对照组明显增多 (P <0 .0 1 ) ,主要分布于浸润的炎症细胞、气道上皮细胞、肺泡上皮细胞、胸膜间皮细胞和血管内皮细胞 .NAC治疗组气道和肺组织中阳性细胞数较LPS致伤组明显减少 (P <0 .0 1 ) ,West ern blot结果验证了上述结果 .结论 :LPS诱发的大鼠急性肺损伤模型中 ,磷酸化p38MAPK在气道和肺组织内表达增加 ,p38MAPK的激活见于肺组织内多数细胞 ,提示肺内炎性和非炎性细胞均有p38MAPK信号分子的激活 .NAC是有效的抗氧化剂 。
AIM: To observe the in situ phosphated expression of p38 MAPK in lung tissues of acute lung injury rat model induced by Lipopolysaccharide (LPS). METHODS: We used immunohistochemical ABC and Western blot method to detect the expression of p38 MAPK in lung tissues and airway epithelial cells in acute lung injury rat model and observed the influence of N acetylcysteine (NAC) on the expression. RESULTS: There were rare p38 MAPK positive cells scattered in alveolar and airway epithelial cells in the control group ( P <0.01). The positive p38 MAPK cells in LPS injury groups increased obviously than those in the control groups ( P <0.01), and were mainly distributed in infiltrative inflammatory cells, airway epithelial cells, alveolar epithelial cells and pleura mesepithelial cells. In NAC treatment group, the p38 MAPK positive cells in airway and lung tissue were much less than those in the acute lung injury group ( P <0.05). The Western blot results were consistent with those of immunohistochemical method. CONCLUSION: p38 MAPK expression increases in alveolar and airway epithelial cells in LPS induced acute lung injury rat models. The activation of p38 MAPK is found in most lung tissues, suggesting that p38 MAPK participates in the signal transduction in inflammatory and noninflammatory cells. NAC is an effective antioxidant, which relieves the inflammation in acute lung injury rats, possibly through the inhibition of p38 MAPK activation.
出处
《第四军医大学学报》
北大核心
2004年第15期1353-1355,共3页
Journal of the Fourth Military Medical University
基金
军队青年基金 (0 1Q1 2 5)
陕西省自然科学基金 (2 0 0 3C 2 0 4 0 )
