摘要
目的 探索眼局部应用榄香烯对角膜碱烧伤诱发新生血管的抑制作用 ,以期为榄香烯抗新生血管发生的临床应用提供依据。方法 采用 0 .5mol·L-1氢氧化钠烧伤兔眼角膜诱发新生血管模型。研究分为 2组 :组Ⅰ ,于碱烧伤后第 2天每日结膜下注射榄香烯 2 5mg,连续 5d ,1周后重复给药 ,连续 5d。组Ⅱ ,采用 5 FU结膜下注射 12 .5mg ,给药方法与组Ⅰ同。同时 ,配以非给药造模对照组。应用裂隙灯检查、角膜照相、眼计算机立体分析系统 ,观察并分析新生血管面积 ,每周 1次 ,连续 7周。比较两种药物对新生血管作用的动态变化。另外 ,正常兔、大鼠各 10只结膜下注射榄香烯和 5 FU ,观察毒性作用及耐受情况 ,以期找到安全剂量。结果 眼局部注射榄香烯的毒副作用小 ,重复给药动物反应耐受。有 2 0 %～ 30 %出现眼部刺激、红肿、畏光及角膜浸润。组Ⅰ、组Ⅱ和非给药造模对照组在 7周时角膜新生血管面积分别为 31 .88ｍｍ2 、4 9.84ｍｍ2 和 6 5.2 0ｍｍ2 ,组间比较有显著性统计学差异 (Ｐ <0 .0 5)。结论 本研究结果显示结膜下注射榄香烯和 5 ＦＵ对角膜碱烧伤诱发的新生血管形成有抑制作用 ,榄香烯作用优于 5 ＦＵ。榄香烯对已形成的角膜新生血管也有一定的消退作用。眼局部应用无明显的毒副作用 。
Objective To investigate the efficacy of inhibition on induced by corneal neovascularization induced by with subconjunctival injection of elemene, in order to provide experimental evidence for its clinical application. Methods Corneal neovascularization model was established by putting the filter paper with the size of 3mm in diameter were immerse in 0.5mol·L -1 NaOH solution on the rabbit cornea for 30 seconds. The experimental animals were divided into two groups. Group Ⅰ: daily subconjuctival injection of elemene,2.5 mg for 5 days in the right eye. One week later,another course of subconjunctival injection of elemene was repeated for 5 days. Group Ⅱ:subconjunctival injection of 5-FU 12.5mg in the left eye,the treatment was conducted the same as the right eye. At the same time,no-medication group was compared.Slit-lamp biomicroscopy and photography of the cornea were weekly for 7 weeks. The total neovascularization area on the cornea was calculated by imaging analysis system of the corneal photos. Another group of 10 rabbits and 10 rats was used for evaluating the toxicity effects of subconjunctival injection of elemene and 5-FU in the right and left eyes respectively. Results Mild photophobia and conjunctiva congestion associated with punctuates erosion of corneal epithelium were noted in 20%～30% of rabbits,both in elemene and 5-FU eye and recovered within few days. The neovascularization area was found to be 31.88mm 2,49.84mm 2 and 65.20mm 2, in the elemene-treated eyes and 5-FU treated eyes and compared group ,the difference was highly significant (P<0.05). Conclusion The results of this study demonstrate that both of elemene and 5-FU are effective in the inhibition of corneal neovacularization induced by alkaline burn,however,the former appears to be more effective than the later. In addition,elemene is well tolerated with only few transient mild side effects.
出处
《眼科新进展》
CAS
2004年第4期250-253,共4页
Recent Advances in Ophthalmology
基金
北京市中医药科技发展基金资助 (编号 :2 0 0 1 2 0 0 2 )~~
关键词
榄香烯
角膜
新生血管
elemene
cornea
neovascularization
