摘要
目的 研究人Th2细胞因子对活化的牛主动脉内皮细胞 (BAECs)表达细胞间黏附分子 1(ICAM 1)和E 选择素 (E selectin)的影响 ,为Th2细胞因子应用于减轻排斥反应、诱导异种移植免疫耐受提供实验依据。方法 用不同浓度(2、5、10、2 0、4 0ng/ml)的Th2细胞因子 (白介素 4、白介素 10、白介素 13)分别孵育BAECs2h后 ,再与TNF α(4ng/ml)共孵育 6h或 18h ;应用细胞 酶联免疫吸附法检测细胞表面的E selectin和ICAM 1的表达。用MTT法测定药物对细胞活性的影响。结果 用一定浓度的Th2细胞因子预处理BAECs后 ,能明显抑制TNF α诱导的E selectin与ICAM 1的表达 ,并呈现一定的剂量依赖性。MTT法测定表明 ,各实验组细胞活性与对照组无明显差异。结论 Th2细胞因子能明显抑制TNF α活化的BAECs表达E selectin与ICAM 1;
Objective To investigate the protective effects of Th2 cytokine (hIL-4, hIL-10, hIL-13) on the bovine aortic endothelial cells(BAECs) stimulated by tumor necrosis factor alpher(TNF-α). Methods BAECs were activated by TNF-α(4ng/ml), and a varied dose of Th2 cytokine(2, 5, 10, 20 and 40ng/ml) was used to incubate BAECs for 2h before stimulation with TNF-α, and then co-incubate for 6h or 18h. The expression of cellular adhesive molecules on BAECs was examined by the cellular enzyme-linked immunoabsorbent assay (Cell-ELISA), and BAECs viability was determined by MTT method. Results BAECs pre-treated with Th2 cytokine could down-regulate the expression of E-selectin and ICAM-1 induced by TNF-α in a dose-dependent manner(P<0.01), while Th2 cytokine has no effects on BAECs viability. Conclusion Th2 cytokine can inhibit the expression of E-selectin and ICAM-1 on BAECs induced by TNF-α, which may contribute to the xenotransplant immune tolerance.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第7期622-624,共3页
Medical Journal of Chinese People's Liberation Army
