摘要
2002年10月以来,贵州省8个养羊场相继发生一种以皮肤、呼吸道和消化道黏膜出现痘疹的传染病。通过电镜观察在痘疹材料中发现大量典型的痘病毒粒子;感染鸡胚后绒毛尿囊膜增厚、充血和出现痘斑;接种BHK21单层细胞引起明显的细胞病变效应,而且这种现象能够被山羊痘参考血清所抑制。血清学检测结果表明,患病羊痘疹材料中山羊痘抗原的阳性率为66 7%;血清样本中山羊痘抗体的阳性率为47 4%。根据对本次疫病的病原学研究和血清学检测,可以确诊贵州省山羊痘暴发流行。
An infectious disease has broken out in 8 goat farms of Guizhou province since October,2002.This disease was characterized by pox on skin,respiratory tract and digestive tract of sick goats.Typical virus particles were observed in pox materials by electronic microscope.The infected chorioallantoic membrane became thicken,congestive and pox formation,the affected monolayer of BHK(21) manifested cytopathogenic effect,and all these phenomena could be inhibited by reference serum of goat pox.The results of serological detection showed that the positive rate of tested antigen was 66.7% in pox materials and the positive rate of tested antibody was 47.4% in serum samples.According to the etiological study and serological detection,goat pox has been definitely diagnosed in Guizhou province.
出处
《山地农业生物学报》
2004年第4期300-304,共5页
Journal of Mountain Agriculture and Biology
基金
贵州省科技厅年度攻关资助项目[黔科合农社字(200NGY)007]
关键词
贵州
山羊痘
病原学
血清学
绒毛尿囊膜
goat pox
observation of electronic microscope
chorioallantoic membrane
cytopathogenic effect
serological detection
