摘要
目的 观察不同剂量的三七总甙对体外培养大鼠成骨细胞增殖、分化及OPG表达的影响,探索体外作用的机制和最佳剂量。方法 第2代培养的成骨细胞分别加入终浓度为Oμg/ml、10μg/ml、50μg/ml、100μg/ml的三七总甙,观察细胞生长、钙结节形成情况,碱性磷酸酶(ALP)染色、分泌量检测,骨钙素(OCN)检测,MTT法观察成骨细胞增殖,免疫组化SP法结合阳性细胞计数法检测成骨细胞OPG表达。结果 成骨细胞在7d可铺满瓶壁,30d可形成钙结节,三七总甙可促进成骨细胞的增殖ALP、OCN的分泌,以50μg/ml时作用明显。三七总甙可促进成骨细胞OPG的表达,在50μg/ml时作用明显。结论 三七总甙可促进大鼠成骨细胞的增殖、分化,促进成骨细胞OPG的表达,以50μg/ml时作用明显。
Objective To assess the effect of total sapanins of Panax notoginseng (PNS) on the prolifera-tion, differentiation and osteoprotegerin (OPG) expression of osteoblact and investigate the mechanism of OPG in osteoporosis. Methods Osteoblasts obtained from the calvariae of newborn rats were cutured in medium with dif-ferent concentrations of PNS. Cell proliferation was detected by microscopy and MTT method, Differentiation was determined by measuring alkaline phosphatase(ALP) activity and osteocalcin secretion. OPG expression was quanti-fied by immunohistochemistry. Results PNS in 50 μg/ml obviously increased osteoblast proliferiation, differentia-tion and OPG expression. Conclusion PNS stimulates osteoblast proliferention and differentiation, and promote the expression of OPG
出处
《中国骨质疏松杂志》
CAS
CSCD
2004年第2期239-241,243,共4页
Chinese Journal of Osteoporosis
