摘要
目的 探讨移植静脉中细胞凋亡及相关基因表达的动态变化及意义。方法 建立 10 0只Wistar大鼠自体颈静脉移植于肾下腹主动脉模型。采用原位DNA片断末端标记 (TUNEL)和免疫组织化学法 ,检测移植静脉血管平滑肌细胞 (VSMCs)凋亡及相关基因bcl 2和bax的表达。结果 术后 1～ 8周 ,移植静脉凋亡的VSMCs多于对照静脉 (P<0 .0 1) ;术后 2周为凋亡高峰 ,其凋亡细胞达 ( 2 8.5± 16 .6 ) % ,4～ 8周逐渐下降至 ( 8.1± 2 .8) % ;与对照静脉〔( 0 .5± 0 .2 ) %〕相比差异有显著性意义 (P<0 .0 1)。术后 1～ 2周 ,bcl 2表达增多〔( 2 2 .1± 5 .4 ) %〕 ,与对照组〔( 4 .3± 2 .1) %〕及 4～ 8周组间比较差异有显著性意义 (P<0 .0 1) ;移植后 1～ 6周 ,bax表达显著高于对照组〔( 5 .5± 2 .3) %〕及术后 8周组〔( 8.2± 2 .9) %〕 ,P<0 .0 1。结论 bcl 2与bax基因与移植静脉VSMCs的凋亡密切相关 ;
Objective To study the cell apoptosis and the dynamic expression and significance of apoptosis-related genes in graft veins. Methods A rat experimental model of autogenous graft vein was established by transplanting the right external jugular vein to infrarenal abdominal aorta in 100 Wistar rats. TUNEL and immunohistochemistry were used to detect the apoptosis, the expression of apoptosis related genes bcl 2 and bax in vascular smooth muscle cells (VSMCs) of graft veins. Results Within the 8 weeks after transplantation, the apoptotic VSMCs in the graft veins were much more than those in the control group with the apoptotic rate reaching the peak〔(28.5±16.6)%〕 on the 2nd week and dropping to (8.1±2.8)% during the 4th to 8th week. There was statistical difference compared to the control group 〔(0.5±0.2)%, P <0.01〕. From 1 to 2 weeks, the positive rate of bcl 2 was (22.1±5.4)% which was higher than that of the control group and the 4-8 week group ( P <0.01); from 1 to 6 weeks, the expression of bax was higher than that of the control group 〔(5.5±2.3)%〕 and the postoperative 8th week group 〔(8.2±2.9)%, P <0.01〕.Conclusion bcl 2 and bax protein may be involved in the regulation of apoptosis of VSMCs. Apoptosis of VSMCs may be an important factor in graft remodeling and graft vein stenosis.
出处
《中国普外基础与临床杂志》
CAS
2004年第4期295-299,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
国家自然科学基金资助 (项目编号 :3 0 2 0 0 2 76)~~
