摘要
目的 :研究雄激素受体 (AR)和神经生长因子 (NGF)的表达 ,证实它们在心内神经节共存的可能性。方法 :免疫细胞化学双重标记技术。结果 :切片上观察到 3种细胞 :( 1 )AR单标细胞 ,胞核呈棕褐色 ;( 2 )NGF单标细胞 ,胞浆呈红色 ;( 3 )AR/NGF双标细胞 ,胞核棕褐色 ,胞浆为红色。双标细胞占全部阳性标记细胞的 5 0 %～ 60 %。结论 :AR和NGF可共存于同一大鼠心内神经节细胞 。
Objective: To investigate the expression of androgen receptor (AR)and nerve growth factor(NGF)in the intrinsic cardiac ganglia of rats and to identify the possibility of coexistence of them in the intrinsic cardiac ganglia. Methods: The double labeling immunocytochemical staining technique was used. Results: There were 3 immunoreactive(ir) labeled neurons in the intrinsic cardiac ganglia.The AR ir production showed brown in color,located in the nucleus;the NGF ir production showed red in color,located in the cytoplasm;there were AR/NGF double labeled cells in same section,which accounted for about 50% 60% of the total single and double labeled neurons. Conclusion: AR and NGF can coexist in same rat intrinsic cardiac ganglia,suggesting that there may be complex interactions between androgen and neurotrophic factors in maintaining intrinsic cardiac ganglia neuronal structure and function.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2004年第3期277-279,316,共4页
Chinese Journal of Anatomy
