摘要
用分子克隆方法获得口蹄疫病毒、水泡性口炎病毒、蓝舌病病毒、鹿流行性出血热病毒和赤羽病病毒各一段高度保守的基因片段 ,用芯片点样仪点样到包被过的玻璃片上 ,制备成检测芯片。提取样品中的RNA ,进行反转录和荧光标记后滴加到芯片上进行特异性杂交 ,对杂交结果进行扫描检测 ,可同时诊断上述 5种动物传染病 ,此方法不但快速、准确、敏感 ,而且可同时进行多种病毒的检测 。
A gene chip was developed by preparing highly conserved DNA fragments for five animal infectious disease viruses ( Foot and mouth disease virus, Vesicular stomatitis virus, Bluetongue virus, Epizootic haemorrhagic disease virus of deer, Akabane disease virus ) using molecular cloning methods, and the DNA fragments were spotted on to specially treated glass slides. RNA extracted from samples was reverse transcripted, followed by PCR amplification and labeling with fluorescent dyes. The labeled cDNA and prepared gene chip were subjected to specific hybridization, and the hybridization results were detected by photoscanning. The results showed that this gene chip technique could identify and distinguish the five animal infectious diseases viruses. This method is rapid, sensitive and specific, and can screen and quarantine a large number of animal samples within a very short period of time.
出处
《微生物学报》
CAS
CSCD
北大核心
2004年第4期479-482,i001,共5页
Acta Microbiologica Sinica
基金
国家科技攻关重大专项资助项目 ( 2 0 0 1BA80 4A2 2 )
云南省科技攻关计划重点项目 [云计科技 ( 98) 63 6号 ]
昆明市科技计划项目[昆科 (农 )字 2 0 0 2 0 2 0 0 3 ]~~
关键词
口蹄疫
动物病毒
基因芯片
分子克隆
水泡性口炎病毒
蓝舌病病毒
鹿流行性出血热病毒
Foot and mouth disease virus, Vesicular stomatitis virus, Bluetongue virus, Epizootic haemorrhagic disease virus of deer, Akabane disease virus, Gene chip
